1osp: Difference between revisions

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[[Image:1osp.gif|left|200px]]<br />

[[Image:1osp.gif|left|200px]]<br /><applet load="1osp" size="350" color="white" frame="true" align="right" spinBox="true"

<applet load="1osp" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

caption="1osp, resolution 1.95Å" />

'''CRYSTAL STRUCTURE OF OUTER SURFACE PROTEIN A OF BORRELIA BURGDORFERI COMPLEXED WITH A MURINE MONOCLONAL ANTIBODY FAB'''<br />

==Overview==

OspA (outer surface protein A) is an abundant immunogenic lipoprotein of, the Lyme disease spirochete Borrelia burgdorferi. The crystal structure of, a soluble recombinant form of OspA was solved in a complex with the Fab, fragment of mouse monoclonal antibody 184.1 and refined to a resolution of, 1.9 A. OspA has a repetitive antiparallel beta topology with an unusual, nonglobular region of "freestanding" sheet connecting globular N- and, C-terminal domains. Arrays of residues with alternating charges are a, predominant feature of the folding pattern in the nonglobular region. The, 184.1 epitope overlaps with a well conserved surface in the N-terminal, domain, and a hydrophobic cavity buried in a positively charged cleft in, the C-terminal domain is a potential binding site for an unknown ligand., An exposed variable region on the C-terminal domain of OspA is predicted, to be an important factor in the worldwide effectiveness of OspA-based, vaccines.

OspA (outer surface protein A) is an abundant immunogenic lipoprotein of the Lyme disease spirochete Borrelia burgdorferi. The crystal structure of a soluble recombinant form of OspA was solved in a complex with the Fab fragment of mouse monoclonal antibody 184.1 and refined to a resolution of 1.9 A. OspA has a repetitive antiparallel beta topology with an unusual nonglobular region of "freestanding" sheet connecting globular N- and C-terminal domains. Arrays of residues with alternating charges are a predominant feature of the folding pattern in the nonglobular region. The 184.1 epitope overlaps with a well conserved surface in the N-terminal domain, and a hydrophobic cavity buried in a positively charged cleft in the C-terminal domain is a potential binding site for an unknown ligand. An exposed variable region on the C-terminal domain of OspA is predicted to be an important factor in the worldwide effectiveness of OspA-based vaccines.

==About this Structure==

1OSP is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Borrelia_burgdorferi Borrelia burgdorferi] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1OSP OCA].

1OSP is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Borrelia_burgdorferi Borrelia burgdorferi] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OSP OCA].

==Reference==

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[[Category: Mus musculus]]

[[Category: Protein complex]]

[[Category: Lawson, C.L.]]

[[Category: Lawson, C L.]]

[[Category: Li, H.]]

[[Category: borrelia burgdorferi strain b31]]

[[Category: complex (immunoglobulin/lipoprotein)]]

[[Category: outer surface protein a complexed with fab184.1]]

[[Category: outer surface protein a complexed with fab184 1]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Sun Nov 18 09~~:39:~~26 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:21:08 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1osp.gif|left|200px]]<br />
+[[Image:1osp.gif|left|200px]]<br /><applet load="1osp" size="350" color="white" frame="true" align="right" spinBox="true"
-<applet load="1osp" size="450" color="white" frame="true" align="right" spinBox="true"
 caption="1osp, resolution 1.95&Aring;" />
 '''CRYSTAL STRUCTURE OF OUTER SURFACE PROTEIN A OF BORRELIA BURGDORFERI COMPLEXED WITH A MURINE MONOCLONAL ANTIBODY FAB'''<br />
 ==Overview==
-OspA (outer surface protein A) is an abundant immunogenic lipoprotein of, the Lyme disease spirochete Borrelia burgdorferi. The crystal structure of, a soluble recombinant form of OspA was solved in a complex with the Fab, fragment of mouse monoclonal antibody 184.1 and refined to a resolution of, 1.9 A. OspA has a repetitive antiparallel beta topology with an unusual, nonglobular region of "freestanding" sheet connecting globular N- and, C-terminal domains. Arrays of residues with alternating charges are a, predominant feature of the folding pattern in the nonglobular region. The, 184.1 epitope overlaps with a well conserved surface in the N-terminal, domain, and a hydrophobic cavity buried in a positively charged cleft in, the C-terminal domain is a potential binding site for an unknown ligand., An exposed variable region on the C-terminal domain of OspA is predicted, to be an important factor in the worldwide effectiveness of OspA-based, vaccines.
+OspA (outer surface protein A) is an abundant immunogenic lipoprotein of the Lyme disease spirochete Borrelia burgdorferi. The crystal structure of a soluble recombinant form of OspA was solved in a complex with the Fab fragment of mouse monoclonal antibody 184.1 and refined to a resolution of 1.9 A. OspA has a repetitive antiparallel beta topology with an unusual nonglobular region of "freestanding" sheet connecting globular N- and C-terminal domains. Arrays of residues with alternating charges are a predominant feature of the folding pattern in the nonglobular region. The 184.1 epitope overlaps with a well conserved surface in the N-terminal domain, and a hydrophobic cavity buried in a positively charged cleft in the C-terminal domain is a potential binding site for an unknown ligand. An exposed variable region on the C-terminal domain of OspA is predicted to be an important factor in the worldwide effectiveness of OspA-based vaccines.
 ==About this Structure==
-OSP is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Borrelia_burgdorferi Borrelia burgdorferi] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OSP OCA].
+OSP is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Borrelia_burgdorferi Borrelia burgdorferi] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OSP OCA].
 ==Reference==
@@ Line 15: / Line 14: @@
 [[Category: Mus musculus]]
 [[Category: Protein complex]]
-[[Category: Lawson, C.L.]]
+[[Category: Lawson, C L.]]
 [[Category: Li, H.]]
 [[Category: borrelia burgdorferi strain b31]]
 [[Category: complex (immunoglobulin/lipoprotein)]]
-[[Category: outer surface protein a complexed with fab184.1]]
+[[Category: outer surface protein a complexed with fab184 1]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 18 09:39:26 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:21:08 2008''