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| {{STRUCTURE_2pwt| PDB=2pwt | SCENE= }} | | {{STRUCTURE_2pwt| PDB=2pwt | SCENE= }} |
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| '''Crystal structure of the bacterial ribosomal decoding site complexed with aminoglycoside containing the L-HABA group'''
| | ===Crystal structure of the bacterial ribosomal decoding site complexed with aminoglycoside containing the L-HABA group=== |
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| ==Overview==
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| The crystal structure of the complex between oligonucleotide containing the bacterial ribosomal decoding site (A site) and the synthetic paromomycin analogue 1, which contains the gamma-amino-alpha-hydroxybutyryl (L-haba) group at position N1 of ring II (2-DOS ring), and an ether chain with an O-phenethylaminoethyl group at position C2'' of ring III, is reported. Interestingly, next to the paromomycin analogue 1 specifically bound to the A site, a second molecule of 1 with a different conformation is observed at the crystal packing interface which mimics the A-minor interaction between two bulged-out adenines from the A site and the codon-anticodon stem of the mRNA-tRNA complex. Improved antibacterial activity supports the conclusion that analogue 1 might affect protein synthesis on the ribosome in two different ways: 1) specific binding to the A site forces maintenance of the "on" state with two bulged out adenines, and 2) a new binding mode of 1 to an A-minor motif which stabilizes complex formation between the ribosome and the mRNA-tRNA complex regardless of whether the codon-anticodon stem is of the cognate or near-cognate type. | | The line below this paragraph, {{ABSTRACT_PUBMED_17722211}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 17722211 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_17722211}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Rna]] | | [[Category: Rna]] |
| [[Category: X-ray analysis]] | | [[Category: X-ray analysis]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 13:55:37 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 22:43:11 2008'' |
