1rjc: Difference between revisions
From Proteopedia
Jump to navigationJump to search
New page: left|200px<br /> <applet load="1rjc" size="450" color="white" frame="true" align="right" spinBox="true" caption="1rjc, resolution 1.40Å" /> '''Crystal structure o... |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:1rjc.gif|left|200px]]<br /> | [[Image:1rjc.gif|left|200px]]<br /><applet load="1rjc" size="350" color="white" frame="true" align="right" spinBox="true" | ||
<applet load="1rjc" size=" | |||
caption="1rjc, resolution 1.40Å" /> | caption="1rjc, resolution 1.40Å" /> | ||
'''Crystal structure of the camelid single domain antibody cAb-Lys2 in complex with hen egg white lysozyme'''<br /> | '''Crystal structure of the camelid single domain antibody cAb-Lys2 in complex with hen egg white lysozyme'''<br /> | ||
==Overview== | ==Overview== | ||
A central paradigm in immunology states that successful generation of high | A central paradigm in immunology states that successful generation of high affinity antibodies necessitates an immense primary repertoire of antigen-combining sites. Much of the diversity of this repertoire is provided by varying one antigen binding loop, created by inserting randomly a D (diversity) gene out of a small pool between the V and J genes. It is therefore assumed that any particular D-encoded region surrounded by different V and J regions adopts a different conformation. We have solved the structure of two lysozyme-specific variable domains of heavy-chain antibodies isolated from two strictly unrelated dromedaries. These antibodies recombined identical D gene sequences to different V and J precursors with significant variance in their V(D)J junctions. Despite these large differences, the D-encoded loop segments adopt remarkably identical architectures, thus directing the antibodies toward identical epitopes. Furthermore, a striking convergent maturation process occurred in the V region, adapting both binders for their sub-nanomolar affinity association with lysozyme. Hence, on a structural level, humoral immunity may rely more on well developed maturation and selection systems than on the acquisition of large primary repertoires. | ||
==About this Structure== | ==About this Structure== | ||
1RJC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Camelus_dromedarius Camelus dromedarius] and [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] with PO4 and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] Full crystallographic information is available from [http:// | 1RJC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Camelus_dromedarius Camelus dromedarius] and [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] with <scene name='pdbligand=PO4:'>PO4</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RJC OCA]. | ||
==Reference== | ==Reference== | ||
| Line 17: | Line 16: | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Decanniere, K.]] | [[Category: Decanniere, K.]] | ||
[[Category: Genst, E | [[Category: Genst, E De.]] | ||
[[Category: Ghahroudi, M | [[Category: Ghahroudi, M A.]] | ||
[[Category: Kinne, J.]] | [[Category: Kinne, J.]] | ||
[[Category: Loris, R.]] | [[Category: Loris, R.]] | ||
| Line 31: | Line 30: | ||
[[Category: protein-protein hetero complex]] | [[Category: protein-protein hetero complex]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:51:30 2008'' | ||
