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| {{STRUCTURE_2q2k| PDB=2q2k | SCENE= }} | | {{STRUCTURE_2q2k| PDB=2q2k | SCENE= }} |
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| '''Structure of nucleic-acid binding protein'''
| | ===Structure of nucleic-acid binding protein=== |
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| ==Overview==
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| The stable inheritance of genetic material depends on accurate DNA partition. Plasmids serve as tractable model systems to study DNA segregation because they require only a DNA centromere, a centromere-binding protein and a force-generating ATPase. The centromeres of partition (par) systems typically consist of a tandem arrangement of direct repeats. The best-characterized par system contains a centromere-binding protein called ParR and an ATPase called ParM. In the first step of segregation, multiple ParR proteins interact with the centromere repeats to form a large nucleoprotein complex of unknown structure called the segrosome, which binds ParM filaments. pSK41 ParR binds a centromere consisting of multiple 20-base-pair (bp) tandem repeats to mediate both transcription autoregulation and segregation. Here we report the structure of the pSK41 segrosome revealed in the crystal structure of a ParR-DNA complex. In the crystals, the 20-mer tandem repeats stack pseudo-continuously to generate the full-length centromere with the ribbon-helix-helix (RHH) fold of ParR binding successive DNA repeats as dimer-of-dimers. Remarkably, the dimer-of-dimers assemble in a continuous protein super-helical array, wrapping the DNA about its positive convex surface to form a large segrosome with an open, solenoid-shaped structure, suggesting a mechanism for ParM capture and subsequent plasmid segregation. | | The line below this paragraph, {{ABSTRACT_PUBMED_18097417}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 18097417 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_18097417}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Protein-dna]] | | [[Category: Protein-dna]] |
| [[Category: Segregation]] | | [[Category: Segregation]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 14:12:42 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 03:51:47 2008'' |
