2q3n: Difference between revisions

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[[Image:2q3n.gif|left|200px]]
{{Seed}}
[[Image:2q3n.png|left|200px]]


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{{STRUCTURE_2q3n|  PDB=2q3n  |  SCENE=  }}  
{{STRUCTURE_2q3n|  PDB=2q3n  |  SCENE=  }}  


'''Agglutinin from Abrus Precatorius (APA-I)'''
===Agglutinin from Abrus Precatorius (APA-I)===




==Overview==
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Abrin and agglutinin-I from the seeds of Abrus precatorius are type II ribosome-inactivating proteins that inhibit protein synthesis in eukaryotic cells. The two toxins share a high degree of sequence similarity; however, agglutinin-I is weaker in its activity. We compared the kinetics of protein synthesis inhibition by abrin and agglutinin-I in two different cell lines and found that approximately 200-2000-fold higher concentration of agglutinin-I is needed for the same degree of inhibition. Like abrin, agglutinin-I also induced apoptosis in the cells by triggering the intrinsic mitochondrial pathway, although at higher concentrations as compared with abrin. The reason for the decreased toxicity of agglutinin-I became apparent on the analysis of the crystal structure of agglutinin-I obtained by us in comparison with that of the reported structure of abrin. The overall protein folding of agglutinin-I is similar to that of abrin-a with a single disulfide bond holding the toxic A subunit and the lectin-like B-subunit together, constituting a heterodimer. However, there are significant differences in the secondary structural elements, mostly in the A chain. The substitution of Asn-200 in abrin-a with Pro-199 in agglutinin-I seems to be a major cause for the decreased toxicity of agglutinin-I. This perhaps is not a consequence of any kink formation by a proline residue in the helical segment, as reported by others earlier, but due to fewer interactions that proline can possibly have with the bound substrate.
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{{ABSTRACT_PUBMED_16772301}}


==About this Structure==
==About this Structure==
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[[Category: Immunotoxin]]
[[Category: Immunotoxin]]
[[Category: Ribosome-inactivating protein]]
[[Category: Ribosome-inactivating protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 14:01:14 2008''

Revision as of 11:01, 27 July 2008

File:2q3n.png

Template:STRUCTURE 2q3n

Agglutinin from Abrus Precatorius (APA-I)

Template:ABSTRACT PUBMED 16772301

About this Structure

2Q3N is a Protein complex structure of sequences from Abrus precatorius. This structure supersedes the now removed PDB entry 2amz. Full crystallographic information is available from OCA.

Reference

Structure-function analysis and insights into the reduced toxicity of Abrus precatorius agglutinin I in relation to abrin., Bagaria A, Surendranath K, Ramagopal UA, Ramakumar S, Karande AA, J Biol Chem. 2006 Nov 10;281(45):34465-74. Epub 2006 Jun 13. PMID:16772301

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