1ynt: Difference between revisions

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[[Image:1ynt.gif|left|200px]]<br />

[[Image:1ynt.gif|left|200px]]<br /><applet load="1ynt" size="350" color="white" frame="true" align="right" spinBox="true"

<applet load="1ynt" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

caption="1ynt, resolution 3.100Å" />

'''Structure of the immunodominant epitope displayed by the surface antigen 1 (SAG1) of Toxoplasma gondii complexed to a monoclonal antibody'''<br />

==Overview==

Toxoplasma gondii, the intracellular parasite responsible for, toxoplasmosis infects more than one-third of the world population and can, be life-threatening for fetuses and immunocompromised patients. The, surface protein SAG1 is an important immune target, which provides a, strong immune response against the invasive tachyzoite while the other, forms of the parasite, devoid of SAG1 at their surface, are multiplying., In addition to this role as a "hot spot" decoy, SAG1 is predicted to act, as an adhesin during host-cell attachment through its binding to, proteoglycans. To begin to understand the relationships between SAG1, epitopes and the ligand-binding site, we have solved the crystal structure, of the monomeric form of T.gondii SAG1 complexed to a Fab derived from a, monoclonal antibody raised against tachyzoite particles. This antibody, competes strongly with human Toxoplasma-specific sera, suggesting that its, epitope is part of an immunodominant region present on the surface of, SAG1. The structure reveals that this conformational epitope, located, within the SAG1 N-terminal domain, does not overlap with the proposed, ligand-binding pocket. This study provides the first structural, description of the monomeric form of SAG1, and significant insights into, its dual role of adhesin and immune target during parasite infection.

Toxoplasma gondii, the intracellular parasite responsible for toxoplasmosis infects more than one-third of the world population and can be life-threatening for fetuses and immunocompromised patients. The surface protein SAG1 is an important immune target, which provides a strong immune response against the invasive tachyzoite while the other forms of the parasite, devoid of SAG1 at their surface, are multiplying. In addition to this role as a "hot spot" decoy, SAG1 is predicted to act as an adhesin during host-cell attachment through its binding to proteoglycans. To begin to understand the relationships between SAG1 epitopes and the ligand-binding site, we have solved the crystal structure of the monomeric form of T.gondii SAG1 complexed to a Fab derived from a monoclonal antibody raised against tachyzoite particles. This antibody competes strongly with human Toxoplasma-specific sera, suggesting that its epitope is part of an immunodominant region present on the surface of SAG1. The structure reveals that this conformational epitope, located within the SAG1 N-terminal domain, does not overlap with the proposed ligand-binding pocket. This study provides the first structural description of the monomeric form of SAG1, and significant insights into its dual role of adhesin and immune target during parasite infection.

==About this Structure==

1YNT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Finegoldia_magna Finegoldia magna], [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [http://en.wikipedia.org/wiki/Toxoplasma_gondii Toxoplasma gondii] with CD as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1YNT OCA].

1YNT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Finegoldia_magna Finegoldia magna], [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [http://en.wikipedia.org/wiki/Toxoplasma_gondii Toxoplasma gondii] with <scene name='pdbligand=CD:'>CD</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YNT OCA].

==Reference==

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[[Category: Battail-Poirot, N]]

[[Category: Bossus, M.]]

[[Category: Du, M.H.Le.]]

[[Category: Du, M H.Le.]]

[[Category: Ducancel, F.]]

[[Category: Graille, M.]]

[[Category: Letourneur, O.]]

[[Category: Muller, B.H.]]

[[Category: Muller, B H.]]

[[Category: Rolland, D.]]

[[Category: Sibai, G.]]

[[Category: Stura, E.A.]]

[[Category: Stura, E A.]]

[[Category: CD]]

[[Category: conformational epitope]]

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[[Category: toxoplasma gondii]]

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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:07:16 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1ynt.gif|left|200px]]<br />
+[[Image:1ynt.gif|left|200px]]<br /><applet load="1ynt" size="350" color="white" frame="true" align="right" spinBox="true"
-<applet load="1ynt" size="450" color="white" frame="true" align="right" spinBox="true"
 caption="1ynt, resolution 3.100&Aring;" />
 '''Structure of the immunodominant epitope displayed by the surface antigen 1 (SAG1) of Toxoplasma gondii complexed to a monoclonal antibody'''<br />
 ==Overview==
-Toxoplasma gondii, the intracellular parasite responsible for, toxoplasmosis infects more than one-third of the world population and can, be life-threatening for fetuses and immunocompromised patients. The, surface protein SAG1 is an important immune target, which provides a, strong immune response against the invasive tachyzoite while the other, forms of the parasite, devoid of SAG1 at their surface, are multiplying., In addition to this role as a "hot spot" decoy, SAG1 is predicted to act, as an adhesin during host-cell attachment through its binding to, proteoglycans. To begin to understand the relationships between SAG1, epitopes and the ligand-binding site, we have solved the crystal structure, of the monomeric form of T.gondii SAG1 complexed to a Fab derived from a, monoclonal antibody raised against tachyzoite particles. This antibody, competes strongly with human Toxoplasma-specific sera, suggesting that its, epitope is part of an immunodominant region present on the surface of, SAG1. The structure reveals that this conformational epitope, located, within the SAG1 N-terminal domain, does not overlap with the proposed, ligand-binding pocket. This study provides the first structural, description of the monomeric form of SAG1, and significant insights into, its dual role of adhesin and immune target during parasite infection.
+Toxoplasma gondii, the intracellular parasite responsible for toxoplasmosis infects more than one-third of the world population and can be life-threatening for fetuses and immunocompromised patients. The surface protein SAG1 is an important immune target, which provides a strong immune response against the invasive tachyzoite while the other forms of the parasite, devoid of SAG1 at their surface, are multiplying. In addition to this role as a "hot spot" decoy, SAG1 is predicted to act as an adhesin during host-cell attachment through its binding to proteoglycans. To begin to understand the relationships between SAG1 epitopes and the ligand-binding site, we have solved the crystal structure of the monomeric form of T.gondii SAG1 complexed to a Fab derived from a monoclonal antibody raised against tachyzoite particles. This antibody competes strongly with human Toxoplasma-specific sera, suggesting that its epitope is part of an immunodominant region present on the surface of SAG1. The structure reveals that this conformational epitope, located within the SAG1 N-terminal domain, does not overlap with the proposed ligand-binding pocket. This study provides the first structural description of the monomeric form of SAG1, and significant insights into its dual role of adhesin and immune target during parasite infection.
 ==About this Structure==
-YNT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Finegoldia_magna Finegoldia magna], [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [http://en.wikipedia.org/wiki/Toxoplasma_gondii Toxoplasma gondii] with CD as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1YNT OCA].
+YNT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Finegoldia_magna Finegoldia magna], [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [http://en.wikipedia.org/wiki/Toxoplasma_gondii Toxoplasma gondii] with <scene name='pdbligand=CD:'>CD</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YNT OCA].
 ==Reference==
@@ Line 18: / Line 17: @@
 [[Category: Battail-Poirot, N]]
 [[Category: Bossus, M.]]
-[[Category: Du, M.H.Le.]]
+[[Category: Du, M H.Le.]]
 [[Category: Ducancel, F.]]
 [[Category: Graille, M.]]
 [[Category: Letourneur, O.]]
-[[Category: Muller, B.H.]]
+[[Category: Muller, B H.]]
 [[Category: Rolland, D.]]
 [[Category: Sibai, G.]]
-[[Category: Stura, E.A.]]
+[[Category: Stura, E A.]]
 [[Category: CD]]
 [[Category: conformational epitope]]
@@ Line 32: / Line 31: @@
 [[Category: toxoplasma gondii]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 18 09:45:38 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:07:16 2008''