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| {{STRUCTURE_2qd0| PDB=2qd0 | SCENE= }} | | {{STRUCTURE_2qd0| PDB=2qd0 | SCENE= }} |
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| '''Crystal structure of mitoNEET'''
| | ===Crystal structure of mitoNEET=== |
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| ==Overview==
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| MitoNEET is a protein of unknown function present in the mitochondrial membrane that was recently shown to bind specifically the antidiabetic drug pioglizatone. Here, we report the crystal structure of the soluble domain (residues 32-108) of human mitoNEET at 1.8-A resolution. The structure reveals an intertwined homodimer, and each subunit was observed to bind a [2Fe-2S] cluster. The [2Fe-2S] ligation pattern of three cysteines and one histidine differs from the known pattern of four cysteines in most cases or two cysteines and two histidines as observed in Rieske proteins. The [2Fe-2S] cluster is packed in a modular structure formed by 17 consecutive residues. The cluster-binding motif is conserved in at least seven distinct groups of proteins from bacteria, archaea, and eukaryotes, which show a consensus sequence of (hb)-C-X(1)-C-X(2)-(S/T)-X(3)-P-(hb)-C-D-X(2)-H, where hb represents a hydrophobic residue; we term this a CCCH-type [2Fe-2S] binding motif. The nine conserved residues in the motif contribute to iron ligation and structure stabilization. UV-visible absorption spectra indicated that mitoNEET can exist in oxidized and reduced states. Our study suggests an electron transfer function for mitoNEET and for other proteins containing the CCCH motif.
| | The line below this paragraph, {{ABSTRACT_PUBMED_17766439}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 17766439 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_17766439}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Iron-sulfur cluster]] | | [[Category: Iron-sulfur cluster]] |
| [[Category: Metal binding protein]] | | [[Category: Metal binding protein]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 14:45:14 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 13:16:01 2008'' |
