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| {{STRUCTURE_2qdq| PDB=2qdq | SCENE= }} | | {{STRUCTURE_2qdq| PDB=2qdq | SCENE= }} |
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| '''Crystal structure of the talin dimerisation domain'''
| | ===Crystal structure of the talin dimerisation domain=== |
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| ==Overview==
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| Talin is a large dimeric protein that couples integrins to cytoskeletal actin. Here, we report the structure of the C-terminal actin-binding domain of talin, the core of which is a five-helix bundle linked to a C-terminal helix responsible for dimerisation. The NMR structure of the bundle reveals a conserved surface-exposed hydrophobic patch surrounded by positively charged groups. We have mapped the actin-binding site to this surface and shown that helix 1 on the opposite side of the bundle negatively regulates actin binding. The crystal structure of the dimerisation helix reveals an antiparallel coiled-coil with conserved residues clustered on the solvent-exposed face. Mutagenesis shows that dimerisation is essential for filamentous actin (F-actin) binding and indicates that the dimerisation helix itself contributes to binding. We have used these structures together with small angle X-ray scattering to derive a model of the entire domain. Electron microscopy provides direct evidence for binding of the dimer to F-actin and indicates that it binds to three monomers along the long-pitch helix of the actin filament.
| | The line below this paragraph, {{ABSTRACT_PUBMED_18157087}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 18157087 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_18157087}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Structural protein]] | | [[Category: Structural protein]] |
| [[Category: Talin]] | | [[Category: Talin]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 14:46:55 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 14:34:07 2008'' |
