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| [[Image:2qtx.jpg|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_2qtx| PDB=2qtx | SCENE= }} | | {{STRUCTURE_2qtx| PDB=2qtx | SCENE= }} |
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| '''Crystal structure of an Hfq-like protein from Methanococcus jannaschii'''
| | ===Crystal structure of an Hfq-like protein from Methanococcus jannaschii=== |
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| ==Overview==
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| The Sm and Sm-like proteins are conserved in all three domains of life and have emerged as important players in many different RNA-processing reactions. Their proposed role is to mediate RNA-RNA and/or RNA-protein interactions. In marked contrast to eukaryotes, bacteria appear to contain only one distinct Sm-like protein belonging to the Hfq family of proteins. Similarly, there are generally only one or two subtypes of Sm-related proteins in archaea, but at least one archaeon, Methanococcus jannaschii, encodes a protein that is related to Hfq. This archaeon does not contain any gene encoding a conventional archaeal Sm-type protein, suggesting that Hfq proteins and archaeal Sm-homologs can complement each other functionally. Here, we report the functional characterization of M. jannaschii Hfq and its crystal structure at 2.5 A resolution. The protein forms a hexameric ring. The monomer fold, as well as the overall structure of the complex is similar to that found for the bacterial Hfq proteins. However, clear differences are seen in the charge distribution on the distal face of the ring, which is unusually negative in M. jannaschii Hfq. Moreover, owing to a very short N-terminal alpha-helix, the overall diameter of the archaeal Hfq hexamer is significantly smaller than its bacterial counterparts. Functional analysis reveals that Escherichia coli and M. jannaschii Hfqs display very similar biochemical and biological properties. It thus appears that the archaeal and bacterial Hfq proteins are largely functionally interchangeable. | | The line below this paragraph, {{ABSTRACT_PUBMED_17959927}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 17959927 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_17959927}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Srna]] | | [[Category: Srna]] |
| [[Category: Translational regulation]] | | [[Category: Translational regulation]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 15:40:36 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 23:21:05 2008'' |
