2qxf: Difference between revisions

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'''Product bound structure of exonuclease I at 1.5 angstrom resolution'''

==Overview==

In Escherichia coli, exonuclease I (ExoI) is a monomeric processive 3'-5' exonuclease that degrades single-stranded DNA. The enzyme has been implicated as primarily being involved in repairing frameshift mutations. The structure of the enzyme has previously been determined in a hexagonal space group at 2.4 A resolution. Here, the structure of ExoI in complex with a nucleotide product, thymidine 5'-monophosphate, is described in an orthorhombic space group at 1.5 A resolution. This new high-resolution structure provides some insight into the interactions involved in binding a nucleotide product. The conserved active site contains a unique metal-binding position when compared with orthologous sites in the Klenow fragment, T4 DNA polymerase and dnaQ. This unique difference is proposed to be a consequence of the repositioning of an important histidine, His181, away from the active site.

==About this Structure==

2QXF is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k12 Escherichia coli k12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QXF OCA].

==Reference==

Structure of Escherichia coli exonuclease I in complex with thymidine 5'-monophosphate., Busam RD, Acta Crystallogr D Biol Crystallogr. 2008 Feb;64(Pt 2):206-10. Epub 2008, Jan 16. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18219121 18219121]

[[Category: Escherichia coli k12]]

[[Category: Exodeoxyribonuclease I]]

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[[Category: Product bound structure]]

[[Category: Sh3-like domain]]

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@@ Line 11: / Line 11: @@
 '''Product bound structure of exonuclease I at 1.5 angstrom resolution'''
+==Overview==
+In Escherichia coli, exonuclease I (ExoI) is a monomeric processive 3'-5' exonuclease that degrades single-stranded DNA. The enzyme has been implicated as primarily being involved in repairing frameshift mutations. The structure of the enzyme has previously been determined in a hexagonal space group at 2.4 A resolution. Here, the structure of ExoI in complex with a nucleotide product, thymidine 5'-monophosphate, is described in an orthorhombic space group at 1.5 A resolution. This new high-resolution structure provides some insight into the interactions involved in binding a nucleotide product. The conserved active site contains a unique metal-binding position when compared with orthologous sites in the Klenow fragment, T4 DNA polymerase and dnaQ. This unique difference is proposed to be a consequence of the repositioning of an important histidine, His181, away from the active site.
 ==About this Structure==
 QXF is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k12 Escherichia coli k12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QXF OCA].
+==Reference==
+Structure of Escherichia coli exonuclease I in complex with thymidine 5'-monophosphate., Busam RD, Acta Crystallogr D Biol Crystallogr. 2008 Feb;64(Pt 2):206-10. Epub 2008, Jan 16. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18219121 18219121]
 [[Category: Escherichia coli k12]]
 [[Category: Exodeoxyribonuclease I]]
@@ Line 27: / Line 33: @@
 [[Category: Product bound structure]]
 [[Category: Sh3-like domain]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 15:51:50 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 11 10:56:07 2008''