2ra6: Difference between revisions

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[[Image:2ra6.jpg|left|200px]]
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{{STRUCTURE_2ra6|  PDB=2ra6  |  SCENE=  }}  
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'''Crystal Structure of the Possum Milk Whey Lipocalin Trichosurin at pH 4.6 with Bound 4-ethylphenol'''
===Crystal Structure of the Possum Milk Whey Lipocalin Trichosurin at pH 4.6 with Bound 4-ethylphenol===




==Overview==
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Lipocalins are extracellular proteins (17-25 kDa) that bind and transport small lipophilic molecules. The three-dimensional structure of the first lipocalin from a metatherian has been determined at different values of pH both with and without bound ligands. Trichosurin, a protein from the milk whey of the common brushtail possum, Trichosurus vulpecula, has been recombinantly expressed in Escherichia coli, refolded from inclusion bodies, purified and crystallized at two different pH values. The three-dimensional structure of trichosurin was solved by X-ray crystallography in two different crystal forms to 1.9 A (1 A=0.1 nm) and 2.6 A resolution, from crystals grown at low and high pH values respectively. Trichosurin has the typical lipocalin fold, an eight-stranded anti-parallel beta-barrel but dimerizes in an orientation that has not been seen previously. The putative binding pocket in the centre of the beta-barrel is well-defined in both high and low pH structures and is occupied by water molecules along with isopropanol molecules from the crystallization medium. Trichosurin was also co-crystallized with a number of small molecule ligands and structures were determined with 2-naphthol and 4-ethylphenol bound in the centre of the beta-barrel. The binding of phenolic compounds by trichosurin provides clues to the function of this important marsupial milk protein, which is highly conserved across metatherians.
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==About this Structure==
==About this Structure==
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[[Category: Transport]]
[[Category: Transport]]
[[Category: Transport protein]]
[[Category: Transport protein]]
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Revision as of 06:07, 29 July 2008

File:2ra6.png

Template:STRUCTURE 2ra6

Crystal Structure of the Possum Milk Whey Lipocalin Trichosurin at pH 4.6 with Bound 4-ethylphenol

Template:ABSTRACT PUBMED 17685895

About this Structure

2RA6 is a Single protein structure of sequence from Trichosurus vulpecula. Full crystallographic information is available from OCA.

Reference

Three-dimensional structure and ligand binding properties of trichosurin, a metatherian lipocalin from the milk whey of the common brushtail possum Trichosurus vulpecula., Watson RP, Demmer J, Baker EN, Arcus VL, Biochem J. 2007 Nov 15;408(1):29-38. PMID:17685895

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