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| {{STRUCTURE_2ram| PDB=2ram | SCENE= }} | | {{STRUCTURE_2ram| PDB=2ram | SCENE= }} |
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| '''A NOVEL DNA RECOGNITION MODE BY NF-KB P65 HOMODIMER'''
| | ===A NOVEL DNA RECOGNITION MODE BY NF-KB P65 HOMODIMER=== |
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| ==Overview==
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| The crystal structure of the NF-kappa B p65 (RelA) homodimer in complex with a DNA target has been determined to 2.4 A resolution. The two p65 subunits are not symmetrically disposed on the DNA target. The homodimer should optimally bind to a pseudo-palindromic nine base pair target with each subunit recognizing a 5'GGAA-3' half site separated by a central A-T base pair. However, one of the subunits (subunit B) encounters a half site of 5'-GAAA-3'. The single base-pair change from G-C to A-T results in highly unfavorable interactions between this half site and the base contacting protein residues in subunit B, which leads to an 18 degrees rotation of the N-terminal terminal domain from its normal conformation. Remarkably, subunit B retains all the interactions with the sugar phosphate backbone of the DNA target. This mode of interaction allows the NF-kappa B p65 homodimer to recognize DNA targets containing only one cognate half site. Differences in the sequence of the other half site provide variations in conformation and affinity of the complex. | | The line below this paragraph, {{ABSTRACT_PUBMED_9437432}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 9437432 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_9437432}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Phosphorylation]] | | [[Category: Phosphorylation]] |
| [[Category: Transcription regulation]] | | [[Category: Transcription regulation]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 16:33:26 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 14:01:25 2008'' |
