2rdg: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older editNewer edit →
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,328,572 edits
No edit summary
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,328,572 edits
No edit summary
Line 1: Line 1:
[[Image:2rdg.jpg|left|200px]]
{{Seed}}
[[Image:2rdg.png|left|200px]]


<!--
<!--
Line 9: Line 10:
{{STRUCTURE_2rdg|  PDB=2rdg  |  SCENE=  }}  
{{STRUCTURE_2rdg|  PDB=2rdg  |  SCENE=  }}  


'''Crystal structure of Staphylococcal Superantigen-Like protein 11 in complex with Sialyl Lewis X'''
===Crystal structure of Staphylococcal Superantigen-Like protein 11 in complex with Sialyl Lewis X===




==Overview==
<!--
Staphylococcus aureus is a major pathogen that produces a family of 14 staphylococcal superantigen-like (SSL) proteins, which are structurally similar to superantigens but do not stimulate T cells. SSL11 is one member of the family that is found in all staphylococcal strains. Recombinant SSL11 bound to granulocytes and monocytes through a sialic acid-dependent mechanism and was rapidly internalized. SSL11 also bound to sialic acid-containing glycoproteins, such as the Fc receptor for IgA (FcalphaRI) and P-selectin glycoprotein ligand-1 (PSGL-1), and inhibited neutrophil attachment to a P-selectin-coated surface. Biosensor analysis of two SSL11 alleles binding to sialyl Lewis X [sLe(x)- Neu5Acalpha2-3Galbeta1-4(Fuc1-3)GlcNAc] coupled to bovine serum albumin gave dissociation constants of 0.7 and 7 mum respectively. Binding of SSL11 to a glycan array revealed specificity for glycans containing the trisaccharide sialyllactosamine (sLacNac - Neu5Acalpha2-3Galbeta1-4GlcNAc). A 1.6 A resolution crystal structure of SSL11 complexed with sLe(x) revealed a discrete binding site in the C-terminal beta-grasp domain, with predominant interactions with the sialic acid and galactose residues. A single amino acid mutation in the carbohydrate binding site abolished all SSL11 binding. Thus, SSL11 is a staphylococcal protein that targets myeloid cells by binding sialyllactosamine-containing glycoproteins.
The line below this paragraph, {{ABSTRACT_PUBMED_18045383}}, adds the Publication Abstract to the page
(as it appears on PubMed at http://www.pubmed.gov), where 18045383 is the PubMed ID number.
-->
{{ABSTRACT_PUBMED_18045383}}


==About this Structure==
==About this Structure==
Line 32: Line 36:
[[Category: Protein-carbohydrate complex]]
[[Category: Protein-carbohydrate complex]]
[[Category: Toxin]]
[[Category: Toxin]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 16:41:17 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 12:04:07 2008''

Revision as of 09:04, 28 July 2008

File:2rdg.png

Template:STRUCTURE 2rdg

Crystal structure of Staphylococcal Superantigen-Like protein 11 in complex with Sialyl Lewis X

Template:ABSTRACT PUBMED 18045383

About this Structure

2RDG is a Single protein structure of sequence from Staphylococcus aureus. Full crystallographic information is available from OCA.

Reference

The crystal structure of staphylococcal superantigen-like protein 11 in complex with sialyl Lewis X reveals the mechanism for cell binding and immune inhibition., Chung MC, Wines BD, Baker H, Langley RJ, Baker EN, Fraser JD, Mol Microbiol. 2007 Dec;66(6):1342-55. PMID:18045383

Page seeded by OCA on Mon Jul 28 12:04:07 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "https://proteopedia.org/index.php?title=2rdg&oldid=697631"