16vp: Difference between revisions

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[[Image:16vp.gif|left|200px]]<br /><applet load="16vp" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:16vp.gif|left|200px]]<br /><applet load="16vp" size="350" color="white" frame="true" align="right" spinBox="true"

caption="16vp, resolution 2.1Å" />

'''CONSERVED CORE OF THE HERPES SIMPLEX VIRUS TRANSCRIPTIONAL REGULATORY PROTEIN VP16'''<br />

==Overview==

On infection, the herpes simplex virus (HSV) virion protein VP16 (Vmw65;, alphaTIF) forms a transcriptional regulatory complex-the VP16-induced, complex-with two cellular proteins, HCF and Oct-1, on VP16-responsive, cis-regulatory elements in HSV immediate-early promoters called TAATGARAT., Comparison of different HSV VP16 sequences reveals a conserved core region, that is sufficient for VP16-induced complex formation. The crystal, structure of the VP16 core has been determined at 2.1 A resolution. The, results reveal a novel, seat-like protein structure. Together with the, activity of mutant VP16 proteins, the structure of free VP16 suggests that, it contains (1) a disordered carboxy-terminal region that associates with, HCF, Oct-1, and DNA in the VP16-induced complex, and (2) a structured, region involved in virion assembly and possessing a novel DNA-binding, surface that differentiates among TAATGARAT VP16-response elements.

On infection, the herpes simplex virus (HSV) virion protein VP16 (Vmw65; alphaTIF) forms a transcriptional regulatory complex-the VP16-induced complex-with two cellular proteins, HCF and Oct-1, on VP16-responsive cis-regulatory elements in HSV immediate-early promoters called TAATGARAT. Comparison of different HSV VP16 sequences reveals a conserved core region that is sufficient for VP16-induced complex formation. The crystal structure of the VP16 core has been determined at 2.1 A resolution. The results reveal a novel, seat-like protein structure. Together with the activity of mutant VP16 proteins, the structure of free VP16 suggests that it contains (1) a disordered carboxy-terminal region that associates with HCF, Oct-1, and DNA in the VP16-induced complex, and (2) a structured region involved in virion assembly and possessing a novel DNA-binding surface that differentiates among TAATGARAT VP16-response elements.

==About this Structure==

16VP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Human_herpesvirus_6 Human herpesvirus 6] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=16VP OCA].

16VP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Human_herpesvirus_6 Human herpesvirus 6] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=16VP OCA].

==Reference==

Line 16:

[[Category: Gong, W.]]

[[Category: Herr, W.]]

[[Category: Huang, C.C.]]

[[Category: Huang, C C.]]

[[Category: Liu, Y.]]

[[Category: SO4]]

[[Category: transcriptional regulatory protein]]

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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:38:45 2008''

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@@ Line 1: / Line 1: @@
-[[Image:16vp.gif|left|200px]]<br /><applet load="16vp" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:16vp.gif|left|200px]]<br /><applet load="16vp" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="16vp, resolution 2.1&Aring;" />
 '''CONSERVED CORE OF THE HERPES SIMPLEX VIRUS TRANSCRIPTIONAL REGULATORY PROTEIN VP16'''<br />
 ==Overview==
-On infection, the herpes simplex virus (HSV) virion protein VP16 (Vmw65;, alphaTIF) forms a transcriptional regulatory complex-the VP16-induced, complex-with two cellular proteins, HCF and Oct-1, on VP16-responsive, cis-regulatory elements in HSV immediate-early promoters called TAATGARAT., Comparison of different HSV VP16 sequences reveals a conserved core region, that is sufficient for VP16-induced complex formation. The crystal, structure of the VP16 core has been determined at 2.1 A resolution. The, results reveal a novel, seat-like protein structure. Together with the, activity of mutant VP16 proteins, the structure of free VP16 suggests that, it contains (1) a disordered carboxy-terminal region that associates with, HCF, Oct-1, and DNA in the VP16-induced complex, and (2) a structured, region involved in virion assembly and possessing a novel DNA-binding, surface that differentiates among TAATGARAT VP16-response elements.
+On infection, the herpes simplex virus (HSV) virion protein VP16 (Vmw65; alphaTIF) forms a transcriptional regulatory complex-the VP16-induced complex-with two cellular proteins, HCF and Oct-1, on VP16-responsive cis-regulatory elements in HSV immediate-early promoters called TAATGARAT. Comparison of different HSV VP16 sequences reveals a conserved core region that is sufficient for VP16-induced complex formation. The crystal structure of the VP16 core has been determined at 2.1 A resolution. The results reveal a novel, seat-like protein structure. Together with the activity of mutant VP16 proteins, the structure of free VP16 suggests that it contains (1) a disordered carboxy-terminal region that associates with HCF, Oct-1, and DNA in the VP16-induced complex, and (2) a structured region involved in virion assembly and possessing a novel DNA-binding surface that differentiates among TAATGARAT VP16-response elements.
 ==About this Structure==
-VP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Human_herpesvirus_6 Human herpesvirus 6] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=16VP OCA].
+VP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Human_herpesvirus_6 Human herpesvirus 6] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=16VP OCA].
 ==Reference==
@@ Line 16: / Line 16: @@
 [[Category: Gong, W.]]
 [[Category: Herr, W.]]
-[[Category: Huang, C.C.]]
+[[Category: Huang, C C.]]
 [[Category: Liu, Y.]]
 [[Category: SO4]]
 [[Category: transcriptional regulatory protein]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 10:29:21 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:38:45 2008''