1a0p: Difference between revisions

[[Image:1a0p.jpg|left|200px]]<br /><applet load="1a0p" size="350" color="white" frame="true" align="right" spinBox="true"  

The structure of the site-specific recombinase, XerD, that functions in circular chromosome separation, has been solved at 2.5 A resolution and reveals that the protein comprises two domains. The C-terminal domain contains two conserved sequence motifs that are located in similar positions in the structures of XerD, lambda and HP1 integrases. However, the extreme C-terminal regions of the three proteins, containing the active site tyrosine, are very different. In XerD, the arrangement of active site residues supports a cis cleavage mechanism. Biochemical evidence for DNA bending is encompassed in a model that accommodates extensive biochemical and genetic data, and in which the DNA is wrapped around an alpha-helix in a manner similar to that observed for CAP complexed with DNA.

1A0P is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A0P OCA].  

[[Category: Arciszewska, L K.]]

[[Category: Baker, R A.]]

[[Category: Bird, L E.]]

[[Category: Sherratt, D J.]]

[[Category: Subramanya, H S.]]

[[Category: Wigley, D B.]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:39:37 2008''