1a0t: Difference between revisions

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[[Image:1a0t.gif|left|200px]]<br /><applet load="1a0t" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1a0t.gif|left|200px]]<br /><applet load="1a0t" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1a0t, resolution 2.4Å" />

'''SUCROSE-SPECIFIC PORIN, WITH BOUND SUCROSE MOLECULES'''<br />

==Overview==

The X-ray structure of a sucrose-specific porin (ScrY) from Salmonella, typhimurium has been determined by multiple isomorphous replacement at 2.4, A resolution both in its uncomplexed form and with bound sucrose. ScrY is, a noncrystallographic trimer of identical subunits, each with 413, structurally well-defined amino acids. A monomer is built up of 18, anti-parallel beta-strands surrounding a hydrophilic pore, with a topology, closely similar to that of maltoporin. Two non-overlapping sucrose-binding, sites were identified in difference Fourier maps. The higher permeability, for sucrose of ScrY as compared to maltoporin is mainly accounted for by, differences in their pore-lining residues.

The X-ray structure of a sucrose-specific porin (ScrY) from Salmonella typhimurium has been determined by multiple isomorphous replacement at 2.4 A resolution both in its uncomplexed form and with bound sucrose. ScrY is a noncrystallographic trimer of identical subunits, each with 413 structurally well-defined amino acids. A monomer is built up of 18 anti-parallel beta-strands surrounding a hydrophilic pore, with a topology closely similar to that of maltoporin. Two non-overlapping sucrose-binding sites were identified in difference Fourier maps. The higher permeability for sucrose of ScrY as compared to maltoporin is mainly accounted for by differences in their pore-lining residues.

==About this Structure==

1A0T is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium] with SUC and CA as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1A0T OCA].

1A0T is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium] with <scene name='pdbligand=SUC:'>SUC</scene> and <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A0T OCA].

==Reference==

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[[Category: porin]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Tue Nov 20 10~~:32:~~24 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:39:36 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1a0t.gif|left|200px]]<br /><applet load="1a0t" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1a0t.gif|left|200px]]<br /><applet load="1a0t" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1a0t, resolution 2.4&Aring;" />
 '''SUCROSE-SPECIFIC PORIN, WITH BOUND SUCROSE MOLECULES'''<br />
 ==Overview==
-The X-ray structure of a sucrose-specific porin (ScrY) from Salmonella, typhimurium has been determined by multiple isomorphous replacement at 2.4, A resolution both in its uncomplexed form and with bound sucrose. ScrY is, a noncrystallographic trimer of identical subunits, each with 413, structurally well-defined amino acids. A monomer is built up of 18, anti-parallel beta-strands surrounding a hydrophilic pore, with a topology, closely similar to that of maltoporin. Two non-overlapping sucrose-binding, sites were identified in difference Fourier maps. The higher permeability, for sucrose of ScrY as compared to maltoporin is mainly accounted for by, differences in their pore-lining residues.
+The X-ray structure of a sucrose-specific porin (ScrY) from Salmonella typhimurium has been determined by multiple isomorphous replacement at 2.4 A resolution both in its uncomplexed form and with bound sucrose. ScrY is a noncrystallographic trimer of identical subunits, each with 413 structurally well-defined amino acids. A monomer is built up of 18 anti-parallel beta-strands surrounding a hydrophilic pore, with a topology closely similar to that of maltoporin. Two non-overlapping sucrose-binding sites were identified in difference Fourier maps. The higher permeability for sucrose of ScrY as compared to maltoporin is mainly accounted for by differences in their pore-lining residues.
 ==About this Structure==
-A0T is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium] with SUC and CA as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1A0T OCA].
+A0T is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium] with <scene name='pdbligand=SUC:'>SUC</scene> and <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A0T OCA].
 ==Reference==
@@ Line 20: / Line 20: @@
 [[Category: porin]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 10:32:24 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:39:36 2008''