1a5p: Difference between revisions

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[[Image:1a5p.gif|left|200px]]<br /><applet load="1a5p" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1a5p.gif|left|200px]]<br /><applet load="1a5p" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1a5p, resolution 1.6Å" />

'''C[40,95]A VARIANT OF BOVINE PANCREATIC RIBONUCLEASE A'''<br />

==Overview==

The Tyr92-Pro93 peptide group of bovine pancreatic ribonuclease A (RNase, A) exists in the cis conformation in the native state. From, unfolding/refolding kinetic studies of the disulfide-intact wild-type, protein and of a variant in which Pro93 had been replaced by Ala, it had, been suggested that the Tyr92-Ala93 peptide group also exists in the cis, conformation in the native state. Here, we report the crystal structure of, the P93A variant. Although there is disorder in the region of residues 92, and 93, the best structural model contains a cis peptide at this position, lending support to the results of the kinetics experiments. We also report, the crystal structure of the C[40, 95]A variant, which is an analog of the, major rate-determining three-disulfide intermediate in the oxidative, folding of RNase A, missing the 40-95 disulfide bond. As had been detected, by NMR spectroscopy, the crystal structure of this analog shows disorder, in the region surrounding the missing disulfide. However, the global chain, fold of the remainder of the protein, including the disulfide bond between, Cys65 and Cys72, appears to be unaffected by the mutation.

The Tyr92-Pro93 peptide group of bovine pancreatic ribonuclease A (RNase A) exists in the cis conformation in the native state. From unfolding/refolding kinetic studies of the disulfide-intact wild-type protein and of a variant in which Pro93 had been replaced by Ala, it had been suggested that the Tyr92-Ala93 peptide group also exists in the cis conformation in the native state. Here, we report the crystal structure of the P93A variant. Although there is disorder in the region of residues 92 and 93, the best structural model contains a cis peptide at this position, lending support to the results of the kinetics experiments. We also report the crystal structure of the C[40, 95]A variant, which is an analog of the major rate-determining three-disulfide intermediate in the oxidative folding of RNase A, missing the 40-95 disulfide bond. As had been detected by NMR spectroscopy, the crystal structure of this analog shows disorder in the region surrounding the missing disulfide. However, the global chain fold of the remainder of the protein, including the disulfide bond between Cys65 and Cys72, appears to be unaffected by the mutation.

==About this Structure==

1A5P is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5] Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1A5P OCA].

1A5P is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A5P OCA].

==Reference==

Line 14:

[[Category: Pancreatic ribonuclease]]

[[Category: Single protein]]

[[Category: Dodge, R.W.]]

[[Category: Dodge, R W.]]

[[Category: Karplus, P.A.]]

[[Category: Karplus, P A.]]

[[Category: Laity, J.H.]]

[[Category: Laity, J H.]]

[[Category: Pearson, M.A.]]

[[Category: Pearson, M A.]]

[[Category: Scheraga, H.A.]]

[[Category: Scheraga, H A.]]

[[Category: SO4]]

[[Category: hydrolase (nucleic acid]]

[[Category: rna)]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Tue Nov 20 10~~:37:~~37 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:41:09 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1a5p.gif|left|200px]]<br /><applet load="1a5p" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1a5p.gif|left|200px]]<br /><applet load="1a5p" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1a5p, resolution 1.6&Aring;" />
 '''C[40,95]A VARIANT OF BOVINE PANCREATIC RIBONUCLEASE A'''<br />
 ==Overview==
-The Tyr92-Pro93 peptide group of bovine pancreatic ribonuclease A (RNase, A) exists in the cis conformation in the native state. From, unfolding/refolding kinetic studies of the disulfide-intact wild-type, protein and of a variant in which Pro93 had been replaced by Ala, it had, been suggested that the Tyr92-Ala93 peptide group also exists in the cis, conformation in the native state. Here, we report the crystal structure of, the P93A variant. Although there is disorder in the region of residues 92, and 93, the best structural model contains a cis peptide at this position, lending support to the results of the kinetics experiments. We also report, the crystal structure of the C[40, 95]A variant, which is an analog of the, major rate-determining three-disulfide intermediate in the oxidative, folding of RNase A, missing the 40-95 disulfide bond. As had been detected, by NMR spectroscopy, the crystal structure of this analog shows disorder, in the region surrounding the missing disulfide. However, the global chain, fold of the remainder of the protein, including the disulfide bond between, Cys65 and Cys72, appears to be unaffected by the mutation.
+The Tyr92-Pro93 peptide group of bovine pancreatic ribonuclease A (RNase A) exists in the cis conformation in the native state. From unfolding/refolding kinetic studies of the disulfide-intact wild-type protein and of a variant in which Pro93 had been replaced by Ala, it had been suggested that the Tyr92-Ala93 peptide group also exists in the cis conformation in the native state. Here, we report the crystal structure of the P93A variant. Although there is disorder in the region of residues 92 and 93, the best structural model contains a cis peptide at this position, lending support to the results of the kinetics experiments. We also report the crystal structure of the C[40, 95]A variant, which is an analog of the major rate-determining three-disulfide intermediate in the oxidative folding of RNase A, missing the 40-95 disulfide bond. As had been detected by NMR spectroscopy, the crystal structure of this analog shows disorder in the region surrounding the missing disulfide. However, the global chain fold of the remainder of the protein, including the disulfide bond between Cys65 and Cys72, appears to be unaffected by the mutation.
 ==About this Structure==
-A5P is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1A5P OCA].
+A5P is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A5P OCA].
 ==Reference==
@@ Line 14: / Line 14: @@
 [[Category: Pancreatic ribonuclease]]
 [[Category: Single protein]]
-[[Category: Dodge, R.W.]]
+[[Category: Dodge, R W.]]
-[[Category: Karplus, P.A.]]
+[[Category: Karplus, P A.]]
-[[Category: Laity, J.H.]]
+[[Category: Laity, J H.]]
-[[Category: Pearson, M.A.]]
+[[Category: Pearson, M A.]]
-[[Category: Scheraga, H.A.]]
+[[Category: Scheraga, H A.]]
 [[Category: SO4]]
 [[Category: hydrolase (nucleic acid]]
 [[Category: rna)]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 10:37:37 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:41:09 2008''