2v76: Difference between revisions

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[[Image:2v76.jpg|left|200px]]
{{Seed}}
[[Image:2v76.png|left|200px]]


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{{STRUCTURE_2v76|  PDB=2v76  |  SCENE=  }}  
{{STRUCTURE_2v76|  PDB=2v76  |  SCENE=  }}  


'''CRYSTAL STRUCTURE OF THE HUMAN DOK1 PTB DOMAIN'''
===CRYSTAL STRUCTURE OF THE HUMAN DOK1 PTB DOMAIN===




==Overview==
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Integrins play a fundamental role in cell migration and adhesion; knowledge of how they are regulated and controlled is vital for understanding these processes. Recent work showed that Dok1 negatively regulates integrin activation, presumably by competition with talin. To understand how this occurs, we used NMR spectroscopy and X-ray crystallography to investigate the molecular details of interactions with integrins. The binding affinities of beta3 integrin tails for the Dok1 and talin PTB domains were quantified using 15N-1H HSQC titrations, revealing that the unphosphorylated integrin tail binds more strongly to talin than Dok1. Chemical shift mapping showed that unlike talin, Dok1 exclusively interacts with the canonical NPxY motif of the beta3 integrin tail. Upon phosphorylation of Tyr747 in the beta3 integrin tail, however, Dok1 then binds much more strongly than talin. Thus we show that phosphorylation of Tyr747 provides a switch for integrin ligand binding. This switch may represent an in vivo mechanism for control of integrin receptor activation. These results have implications for the control of integrin signaling by proteins containing PTB domains.
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{{ABSTRACT_PUBMED_18156175}}


==About this Structure==
==About this Structure==
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[[Category: Protein-binding]]
[[Category: Protein-binding]]
[[Category: Ptb domain]]
[[Category: Ptb domain]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 18:18:41 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 16:01:26 2008''

Revision as of 13:01, 27 July 2008

File:2v76.png

Template:STRUCTURE 2v76

CRYSTAL STRUCTURE OF THE HUMAN DOK1 PTB DOMAIN

Template:ABSTRACT PUBMED 18156175

About this Structure

2V76 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

An integrin phosphorylation switch: the effect of beta3 integrin tail phosphorylation on DOK1 and talin binding., Oxley CL, Anthis NJ, Lowe ED, Vakonakis I, Campbell ID, Wegener KL, J Biol Chem. 2007 Dec 21;. PMID:18156175

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