2yrf: Difference between revisions

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[[Image:2yrf.~~jpg~~|left|200px]]

[[Image:2yrf.png|left|200px]]

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~~'''~~Crystal structure of 5-methylthioribose 1-phosphate isomerase from Bacillus subtilis complexed with sulfate ion~~'''~~

===Crystal structure of 5-methylthioribose 1-phosphate isomerase from Bacillus subtilis complexed with sulfate ion===

~~==Overview==~~

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The ~~methionine salvage pathway (MSP) plays a crucial role in recycling a sulphahydryl derivative of the nucleoside. Recently~~, the ~~genes and reactions in MSP from Bacillus subtilis have been identified, where 5-methylthioribose 1-phosphate isomerase (M1Pi) catalyzes a conversion of 5-methylthioribose 1-phosphate (MTR-1-P)~~ to ~~5-methylthioribulose 1-phosphate (MTRu-1-P). Herein, we report~~ the ~~crystal structures of B. subtilis M1Pi~~ (~~Bs-M1Pi) in complex with its product MTRu-1-P, and a sulfate~~ at 2.~~4 and 2~~.~~7 A resolution~~, ~~respectively. The electron density clearly shows~~ the ~~presence of each compound in the active site~~. ~~The structural comparison with other homologous proteins explains how the substrate uptake of Bs~~-M1Pi may be induced by an open/closed transition of the active site. The highly conserved residues at the active site, namely, Cys160 and Asp240 are most likely to be involved in catalysis. The structural analysis sheds light on its catalytic mechanism of M1Pi.

The line below this paragraph, {{ABSTRACT_PUBMED_18156470}}, adds the Publication Abstract to the page

(as it appears on PubMed at http://www.pubmed.gov), where 18156470 is the PubMed ID number.

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==About this Structure==

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[[Category: Isomerase]]

[[Category: Methionine salvage pathway]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sun May 4 19~~:15:08 2008''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 01:31:31 2008''

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-[[Image:2yrf.jpg|left|200px]]
+{{Seed}}
+[[Image:2yrf.png|left|200px]]
 <!--
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 {{STRUCTURE_2yrf|  PDB=2yrf  |  SCENE=  }}
-'''Crystal structure of 5-methylthioribose 1-phosphate isomerase from Bacillus subtilis complexed with sulfate ion'''
+===Crystal structure of 5-methylthioribose 1-phosphate isomerase from Bacillus subtilis complexed with sulfate ion===
-==Overview==
+<!--
-The methionine salvage pathway (MSP) plays a crucial role in recycling a sulphahydryl derivative of the nucleoside. Recently, the genes and reactions in MSP from Bacillus subtilis have been identified, where 5-methylthioribose 1-phosphate isomerase (M1Pi) catalyzes a conversion of 5-methylthioribose 1-phosphate (MTR-1-P) to 5-methylthioribulose 1-phosphate (MTRu-1-P). Herein, we report the crystal structures of B. subtilis M1Pi (Bs-M1Pi) in complex with its product MTRu-1-P, and a sulfate at 2.4 and 2.7 A resolution, respectively. The electron density clearly shows the presence of each compound in the active site. The structural comparison with other homologous proteins explains how the substrate uptake of Bs-M1Pi may be induced by an open/closed transition of the active site. The highly conserved residues at the active site, namely, Cys160 and Asp240 are most likely to be involved in catalysis. The structural analysis sheds light on its catalytic mechanism of M1Pi.
+The line below this paragraph, {{ABSTRACT_PUBMED_18156470}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 18156470 is the PubMed ID number.
+-->
+{{ABSTRACT_PUBMED_18156470}}
 ==About this Structure==
@@ Line 30: / Line 34: @@
 [[Category: Isomerase]]
 [[Category: Methionine salvage pathway]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 19:15:08 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 01:31:31 2008''