1af5: Difference between revisions

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[[Image:1af5.gif|left|200px]]<br /><applet load="1af5" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1af5.gif|left|200px]]<br /><applet load="1af5" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1af5, resolution 3.00Å" />

'''GROUP I MOBILE INTRON ENDONUCLEASE'''<br />

==Overview==

The structure of I-Crel provides the first view of a protein encoded by a, gene within an intron. This endonuclease recognizes a long DNA site, approximately 20 base pairs in length and facilitates the lateral transfer, of that intron. The protein exhibits a DNA-binding surface consisting of, four antiparallel beta-strands that form a 20 A wide groove which is over, 70 A long. The architecture of this fold is different from that of the, TATA binding protein, TBP, which also contains an antiparallel, beta-saddle. The conserved LAGLIDADG motif, which is found in many mobile, intron endonucleases, maturases and inteins, forms a novel helical, interface and contributes essential residues to the active site.

The structure of I-Crel provides the first view of a protein encoded by a gene within an intron. This endonuclease recognizes a long DNA site approximately 20 base pairs in length and facilitates the lateral transfer of that intron. The protein exhibits a DNA-binding surface consisting of four antiparallel beta-strands that form a 20 A wide groove which is over 70 A long. The architecture of this fold is different from that of the TATA binding protein, TBP, which also contains an antiparallel beta-saddle. The conserved LAGLIDADG motif, which is found in many mobile intron endonucleases, maturases and inteins, forms a novel helical interface and contributes essential residues to the active site.

==About this Structure==

1AF5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1AF5 OCA].

1AF5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AF5 OCA].

==Reference==

Line 13:

[[Category: Chlamydomonas reinhardtii]]

[[Category: Single protein]]

[[Category: Heath, P.J.]]

[[Category: Heath, P J.]]

[[Category: Junior, R.J.Monnat.]]

[[Category: Junior, R J.Monnat.]]

[[Category: Stephens, K.M.]]

[[Category: Stephens, K M.]]

[[Category: Stoddard, B.L.]]

[[Category: Stoddard, B L.]]

[[Category: chloroplast dna]]

[[Category: endonuclease]]

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[[Category: laglidadg motif]]

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@@ Line 1: / Line 1: @@
-[[Image:1af5.gif|left|200px]]<br /><applet load="1af5" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1af5.gif|left|200px]]<br /><applet load="1af5" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1af5, resolution 3.00&Aring;" />
 '''GROUP I MOBILE INTRON ENDONUCLEASE'''<br />
 ==Overview==
-The structure of I-Crel provides the first view of a protein encoded by a, gene within an intron. This endonuclease recognizes a long DNA site, approximately 20 base pairs in length and facilitates the lateral transfer, of that intron. The protein exhibits a DNA-binding surface consisting of, four antiparallel beta-strands that form a 20 A wide groove which is over, 70 A long. The architecture of this fold is different from that of the, TATA binding protein, TBP, which also contains an antiparallel, beta-saddle. The conserved LAGLIDADG motif, which is found in many mobile, intron endonucleases, maturases and inteins, forms a novel helical, interface and contributes essential residues to the active site.
+The structure of I-Crel provides the first view of a protein encoded by a gene within an intron. This endonuclease recognizes a long DNA site approximately 20 base pairs in length and facilitates the lateral transfer of that intron. The protein exhibits a DNA-binding surface consisting of four antiparallel beta-strands that form a 20 A wide groove which is over 70 A long. The architecture of this fold is different from that of the TATA binding protein, TBP, which also contains an antiparallel beta-saddle. The conserved LAGLIDADG motif, which is found in many mobile intron endonucleases, maturases and inteins, forms a novel helical interface and contributes essential residues to the active site.
 ==About this Structure==
-AF5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1AF5 OCA].
+AF5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AF5 OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Chlamydomonas reinhardtii]]
 [[Category: Single protein]]
-[[Category: Heath, P.J.]]
+[[Category: Heath, P J.]]
-[[Category: Junior, R.J.Monnat.]]
+[[Category: Junior, R J.Monnat.]]
-[[Category: Stephens, K.M.]]
+[[Category: Stephens, K M.]]
-[[Category: Stoddard, B.L.]]
+[[Category: Stoddard, B L.]]
 [[Category: chloroplast dna]]
 [[Category: endonuclease]]
@@ Line 23: / Line 23: @@
 [[Category: laglidadg motif]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 10:48:23 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:43:57 2008''