1ag4: Difference between revisions
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New page: left|200px<br /><applet load="1ag4" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ag4" /> '''NMR STRUCTURE OF SPHERULIN 3A (S3A) FROM PHY... |
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[[Image:1ag4.gif|left|200px]]<br /><applet load="1ag4" size=" | [[Image:1ag4.gif|left|200px]]<br /><applet load="1ag4" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1ag4" /> | caption="1ag4" /> | ||
'''NMR STRUCTURE OF SPHERULIN 3A (S3A) FROM PHYSARUM POLYCEPHALUM, MINIMIZED AVERAGE STRUCTURE'''<br /> | '''NMR STRUCTURE OF SPHERULIN 3A (S3A) FROM PHYSARUM POLYCEPHALUM, MINIMIZED AVERAGE STRUCTURE'''<br /> | ||
==Overview== | ==Overview== | ||
Spherulin 3a is the most abundantly expressed cytosolic protein in | Spherulin 3a is the most abundantly expressed cytosolic protein in spherulating plasmodia of the slime mold Physarum polycephalum. High yields of unlabeled, uniformly 15N and uniformly 13C/15N-labeled recombinant spherulin 3a from Escherichia coli could be produced by a simple protocol described here. The three-dimensional solution structure of Ca2+-loaded spherulin 3a was determined by homo- and heteronuclear NMR spectroscopy. The structure of monomeric spherulin 3a consists of two pleated beta-sheets plus a short alpha-helix arranged into the gamma-crystallin fold. The beta-sheets comprise two intertwined Greek-key motifs. An additional N-terminal beta-strand is unique to spherulin 3a. Complexation of calcium ions greatly enhances overall conformational stability of the protein. The average atomic root-mean-square deviations (r.m.s.d.) for heavy atoms in beta-strands were 0.34(+/-0.16) A for the backbone atoms and 0.73(+/-0.40) A for all atoms. The corresponding r.m.s.d. values for heavy atoms in the whole protein were 0.62(+/-0.42) A for the backbone atoms and 0.99(+/-0.65) A for all atoms. We show the structural relationship between spherulin 3a, a myxomycete dormancy protein, and crystallins from the vertebrate eye lens. Since spherulin 3a has a structure corresponding to one domain of bovine gammaB(II)-crystallin, it represents a hypothetical ancestral gamma-crystallin precursor structure. | ||
==About this Structure== | ==About this Structure== | ||
1AG4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Physarum_polycephalum Physarum polycephalum]. Full crystallographic information is available from [http:// | 1AG4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Physarum_polycephalum Physarum polycephalum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AG4 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Physarum polycephalum]] | [[Category: Physarum polycephalum]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Holak, T | [[Category: Holak, T A.]] | ||
[[Category: Jaenicke, R.]] | [[Category: Jaenicke, R.]] | ||
[[Category: Mayr, E | [[Category: Mayr, E M.]] | ||
[[Category: Renner, C.]] | [[Category: Renner, C.]] | ||
[[Category: Rosinke, B.]] | [[Category: Rosinke, B.]] | ||
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[[Category: structural protein]] | [[Category: structural protein]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:44:05 2008'' | ||
