2zb9: Difference between revisions

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[[Image:2zb9.~~jpg~~|left|200px]]

[[Image:2zb9.png|left|200px]]

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~~'''~~Crystal structure of TetR family transcription regulator SCO0332~~'''~~

===Crystal structure of TetR family transcription regulator SCO0332===

~~==Overview==~~

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~~SCO0332 protein is a putative TetR-family transcriptional regulator from Streptomyces coelicolor A3(2).~~ The ~~crystal structure of SCO0332 was determined at 2.25 A resolution by single-wavelength anomalous diffraction (SAD) phasing using~~ the ~~S atoms of~~ the ~~native protein. SCO0332 contains a helix-turn-helix~~ (~~HTH) DNA-binding motif in its N-terminal region and forms a homodimer~~. ~~The overall structure of SCO0332 shows significant similarity to other TetR-family regulators~~. ~~A systematic evolution of ligands by exponential enrichment (SELEX~~) ~~analysis indicated that SCO0332 has sequence-specific DNA-binding ability and determined the position of the operator element of SCO0332 on~~ the ~~chromosomal DNA of S~~. ~~coelicolor. An electrophoretic mobility~~-~~shift assay (EMSA) showed that SCO0332 binds to the operator sequence upstream of the sco0330 gene, which encodes a putative short~~-~~chain oxidoreductase. These results suggest that SCO0332 is a transcriptional repressor that regulates sco0330 gene expression.~~

The line below this paragraph, {{ABSTRACT_PUBMED_18219120}}, adds the Publication Abstract to the page

(as it appears on PubMed at http://www.pubmed.gov), where 18219120 is the PubMed ID number.

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==About this Structure==

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[[Category: Transcription regulation]]

[[Category: Transcription regulator]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sun May 4~~ 20:06:27 2008''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 20:30:30 2008''

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-[[Image:2zb9.jpg|left|200px]]
+{{Seed}}
+[[Image:2zb9.png|left|200px]]
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 {{STRUCTURE_2zb9|  PDB=2zb9  |  SCENE=  }}
-'''Crystal structure of TetR family transcription regulator SCO0332'''
+===Crystal structure of TetR family transcription regulator SCO0332===
-==Overview==
+<!--
-SCO0332 protein is a putative TetR-family transcriptional regulator from Streptomyces coelicolor A3(2). The crystal structure of SCO0332 was determined at 2.25 A resolution by single-wavelength anomalous diffraction (SAD) phasing using the S atoms of the native protein. SCO0332 contains a helix-turn-helix (HTH) DNA-binding motif in its N-terminal region and forms a homodimer. The overall structure of SCO0332 shows significant similarity to other TetR-family regulators. A systematic evolution of ligands by exponential enrichment (SELEX) analysis indicated that SCO0332 has sequence-specific DNA-binding ability and determined the position of the operator element of SCO0332 on the chromosomal DNA of S. coelicolor. An electrophoretic mobility-shift assay (EMSA) showed that SCO0332 binds to the operator sequence upstream of the sco0330 gene, which encodes a putative short-chain oxidoreductase. These results suggest that SCO0332 is a transcriptional repressor that regulates sco0330 gene expression.
+The line below this paragraph, {{ABSTRACT_PUBMED_18219120}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 18219120 is the PubMed ID number.
+-->
+{{ABSTRACT_PUBMED_18219120}}
 ==About this Structure==
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 [[Category: Transcription regulation]]
 [[Category: Transcription regulator]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 20:06:27 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 20:30:30 2008''