2zcg: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older editNewer edit →
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,331,097 edits
No edit summary
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,331,097 edits
No edit summary
Line 1: Line 1:
[[Image:2zcg.jpg|left|200px]]
{{Seed}}
[[Image:2zcg.png|left|200px]]


<!--
<!--
Line 9: Line 10:
{{STRUCTURE_2zcg|  PDB=2zcg  |  SCENE=  }}  
{{STRUCTURE_2zcg|  PDB=2zcg  |  SCENE=  }}  


'''Structure and inhibition of orotidine 5'-phosphate decarboxylase from plasmodium falciparum'''
===Structure and inhibition of orotidine 5'-phosphate decarboxylase from plasmodium falciparum===




==Overview==
<!--
Orotidine 5'-monophosphate (OMP) decarboxylase from Plasmodium falciparum ( PfODCase, EC 4.1.1.23) has been overexpressed, purified, subjected to kinetic and biochemical analysis, and crystallized. The native enzyme is a homodimer with a subunit molecular mass of 38 kDa. The saturation curve for OMP as a substrate conformed to Michaelis-Menten kinetics with K m = 350 +/- 60 nM and V max = 2.70 +/- 0.10 micromol/min/mg protein. Inhibition patterns for nucleoside 5'-monophosphate analogues were linear competitive with respect to OMP with a decreasing potency of inhibition of PfODCase in the order: pyrazofurin 5'-monophosphate ( K i = 3.6 +/- 0.7 nM) &gt; xanthosine 5'-monophosphate (XMP, K i = 4.4 +/- 0.7 nM) &gt; 6-azauridine 5'-monophosphate (AzaUMP, K i = 12 +/- 3 nM) &gt; allopurinol-3-riboside 5'-monophosphate ( K i = 240 +/- 20 nM). XMP is an approximately 150-fold more potent inhibitor of PfODCase compared with the human enzyme. The structure of PfODCase was solved in the absence of ligand and displays a classic TIM-barrel fold characteristic of the enzyme. Both the phosphate-binding loop and the betaalpha5-loop have conformational flexibility, which may be associated with substrate capture and product release along the reaction pathway.
The line below this paragraph, {{ABSTRACT_PUBMED_18303855}}, adds the Publication Abstract to the page
(as it appears on PubMed at http://www.pubmed.gov), where 18303855 is the PubMed ID number.
-->
{{ABSTRACT_PUBMED_18303855}}


==About this Structure==
==About this Structure==
Line 31: Line 35:
[[Category: Pyrimidine biosynthesis]]
[[Category: Pyrimidine biosynthesis]]
[[Category: Tim barrel]]
[[Category: Tim barrel]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 20:08:40 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 13:35:49 2008''

Revision as of 10:35, 27 July 2008

File:2zcg.png

Template:STRUCTURE 2zcg

Structure and inhibition of orotidine 5'-phosphate decarboxylase from plasmodium falciparum

Template:ABSTRACT PUBMED 18303855

About this Structure

2ZCG is a Single protein structure of sequence from Plasmodium falciparum. Full crystallographic information is available from OCA.

Reference

Structure and Inhibition of Orotidine 5'-Monophosphate Decarboxylase from Plasmodium falciparum., Langley DB, Shojaei M, Chan C, Lok HC, Mackay JP, Traut TW, Guss JM, Christopherson RI, Biochemistry. 2008 Feb 28;. PMID:18303855

Page seeded by OCA on Sun Jul 27 13:35:49 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "https://proteopedia.org/index.php?title=2zcg&oldid=648707"