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| {{STRUCTURE_3bdq| PDB=3bdq | SCENE= }} | | {{STRUCTURE_3bdq| PDB=3bdq | SCENE= }} |
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| '''Room Tempreture Crystal Structure of Sterol Carrier Protein-2 Like-2'''
| | ===Room Tempreture Crystal Structure of Sterol Carrier Protein-2 Like-2=== |
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| ==Overview==
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| Mosquito sterol carrier protein-2 (AeSCP-2) and sterol carrier protein-2-like2 (AeSCP-2L2) are members of the SCP-2 protein family with similar expression profiles in the mosquito life cycle. In an effort to understand how lipids can be transported by different SCP-2 proteins, the three-dimensional crystal structure of AeSCP-2L2 was solved at 1.7 A resolution. AeSCP-2L2 forms a dimer and binds three fatty acids, one of which resides in a position within the internal cavity at a right angle to the others. This first report of ligand-bound dimerized protein in the SCP-2 protein family indicates that the family has a much more divergent mode of interaction with ligands than previously reported. The potential function of AeSCP-2L2 was investigated via in vivo incorporation of [(3)H]cholesterol and [(3)H]palmitic acid. Overexpression of AeSCP-2L2 in mosquito cells leads to an increased uptake of free fatty acid, whereas knockdown of AeSCP-2L2 in adult females decreases the accumulation of free fatty acid in the fat body from a blood meal. In contrast, overexpression or knockdown of AeSCP-2L2 has no effect on cholesterol uptake. Our results suggest that the main function of AeSCP-2L2 is as a general intracellular fatty acid carrier, as opposed to having a dedicated role in cholesterol transport.
| | The line below this paragraph, {{ABSTRACT_PUBMED_18084051}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 18084051 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_18084051}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Lipid transport]] | | [[Category: Lipid transport]] |
| [[Category: Sterol carrier protein]] | | [[Category: Sterol carrier protein]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 20:39:50 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 01:20:16 2008'' |
