3bff: Difference between revisions

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[[Image:3bff.~~jpg~~|left|200px]]

[[Image:3bff.png|left|200px]]

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~~'''~~class A beta-lactamase SED-G238C complexed with faropenem~~'''~~

===class A beta-lactamase SED-G238C complexed with faropenem===

~~==Overview==~~

SED-1, a class A beta-lactamase from Citrobacter sedlakii, is a CTX-M-type extended-spectrum beta-lactamase that has the ability to hydrolyze expanded-spectrum cephalosporins such as cefotaxime. SED-1 and a SED mutant in which Gly238 has been replaced by a cysteine, forming a disulfide bridge with the other Cys residue located at position 69 (SED-G238C), have been crystallized. The crystals belong to the monoclinic space group C2, with unit-cell parameters a = 188.09, b = 73.65, c = 105.41 A, beta = 121.67 degrees for SED-1 and a = 187.64, b = 73.2, c = 103.89 A, beta = 121.89 degrees for the SED-G238C mutant. X-ray diffraction data were collected to maximum resolutions of 2.4 A for SED-1 and 2.0 A for SED-G238C.

==About this Structure==

3BFF is a ~~[[Single protein]]~~ structure of ~~sequence~~ from [http://en.wikipedia.org/wiki/Citrobacter_sedlakii Citrobacter sedlakii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BFF OCA].

3BFF is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Citrobacter_sedlakii Citrobacter sedlakii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BFF OCA].

==Reference==

Crystallization and preliminary X-ray diffraction study of the class A beta-lactamase SED-1 and its mutant SED-G238C from Citrobacter sedlakii., Petrella S, Pernot L, Sougakoff W, Acta Crystallogr D Biol Crystallogr. 2004 Jan;60(Pt 1):125-8. Epub 2003, Dec 18. PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/14684905 14684905]~~

[[Category: Beta-lactamase]]

[[Category: Citrobacter sedlakii]]

~~[[Category: Single protein]]~~

[[Category: Pernot, L.]]

[[Category: Petrella, S.]]

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[[Category: Faropenem]]

[[Category: Hydrolase]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sun May 4 20~~:43~~:00 2008~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 11:31:43 2009''

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-[[Image:3bff.jpg|left|200px]]
+{{Seed}}
+[[Image:3bff.png|left|200px]]
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 {{STRUCTURE_3bff|  PDB=3bff  |  SCENE=  }}
-'''class A beta-lactamase SED-G238C complexed with faropenem'''
+===class A beta-lactamase SED-G238C complexed with faropenem===
-==Overview==
-SED-1, a class A beta-lactamase from Citrobacter sedlakii, is a CTX-M-type extended-spectrum beta-lactamase that has the ability to hydrolyze expanded-spectrum cephalosporins such as cefotaxime. SED-1 and a SED mutant in which Gly238 has been replaced by a cysteine, forming a disulfide bridge with the other Cys residue located at position 69 (SED-G238C), have been crystallized. The crystals belong to the monoclinic space group C2, with unit-cell parameters a = 188.09, b = 73.65, c = 105.41 A, beta = 121.67 degrees for SED-1 and a = 187.64, b = 73.2, c = 103.89 A, beta = 121.89 degrees for the SED-G238C mutant. X-ray diffraction data were collected to maximum resolutions of 2.4 A for SED-1 and 2.0 A for SED-G238C.
 ==About this Structure==
-BFF is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Citrobacter_sedlakii Citrobacter sedlakii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BFF OCA].
+BFF is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Citrobacter_sedlakii Citrobacter sedlakii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BFF OCA].
 ==Reference==
-Crystallization and preliminary X-ray diffraction study of the class A beta-lactamase SED-1 and its mutant SED-G238C from Citrobacter sedlakii., Petrella S, Pernot L, Sougakoff W, Acta Crystallogr D Biol Crystallogr. 2004 Jan;60(Pt 1):125-8. Epub 2003, Dec 18. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14684905 14684905]
+<ref group="xtra">PMID:14684905</ref><references group="xtra"/>
 [[Category: Beta-lactamase]]
 [[Category: Citrobacter sedlakii]]
-[[Category: Single protein]]
 [[Category: Pernot, L.]]
 [[Category: Petrella, S.]]
@@ Line 31: / Line 28: @@
 [[Category: Faropenem]]
 [[Category: Hydrolase]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 20:43:00 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 11:31:43 2009''