3bu4: Difference between revisions

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[[Image:3bu4.jpg|left|200px]]
{{Seed}}
[[Image:3bu4.png|left|200px]]


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{{STRUCTURE_3bu4|  PDB=3bu4  |  SCENE=  }}  
{{STRUCTURE_3bu4|  PDB=3bu4  |  SCENE=  }}  


'''RIBONUCLEASE T1 COMPLEX WITH 2'GMP'''
===RIBONUCLEASE T1 COMPLEX WITH 2'GMP===




==Overview==
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We systematically analyzed the crystallographically determined water molecules of all known structures of RNase T1 and compared them to the ordered solvent in a large number of related microbial nucleases. To assess the crystallographers' impact on the interpretation of the solvent structure, we independently refined five validation structures from diffraction data derived from five isomorphous crystals of RNase T1. We also compared the positions of water molecules found in 11 published isomorphous RNase T1 inhibitor complexes. These data suggest that the positions of most of the waters located on the surface of a protein and that are well-determined in the experimental electron density maps are determined primarily by crystal packing forces. Water molecules with less well-defined electron density are in general unique to one or a small number of crystal structures. Only a small number of the well-defined waters are found to be independent of the crystal environment. These waters have a low accessible surface area and B-factor, and tend to be conserved in the crystal structures of a number of evolutionary related ribonucleases as well. A single water molecule is found conserved in all known microbial ribonucleases.
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{{ABSTRACT_PUBMED_10373011}}


==About this Structure==
==About this Structure==
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[[Category: Transue, T R.]]
[[Category: Transue, T R.]]
[[Category: Endoribonuclease]]
[[Category: Endoribonuclease]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 21:06:12 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 10:51:37 2008''

Revision as of 07:51, 28 July 2008

File:3bu4.png

Template:STRUCTURE 3bu4

RIBONUCLEASE T1 COMPLEX WITH 2'GMP

Template:ABSTRACT PUBMED 10373011

About this Structure

3BU4 is a Single protein structure of sequence from Aspergillus oryzae. Full crystallographic information is available from OCA.

Reference

Conserved water molecules in a large family of microbial ribonucleases., Loris R, Langhorst U, De Vos S, Decanniere K, Bouckaert J, Maes D, Transue TR, Steyaert J, Proteins. 1999 Jul 1;36(1):117-34. PMID:10373011

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