3c0n: Difference between revisions

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[[Image:3c0n.~~jpg~~|left|200px]]

[[Image:3c0n.png|left|200px]]

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~~'''~~Crystal structure of the proaerolysin mutant Y221G at 2.2 A~~'''~~

===Crystal structure of the proaerolysin mutant Y221G at 2.2 A===

~~==Overview==~~

Aerolysin is chiefly responsible for the pathogenicity of Aeromonas hydrophila, a bacterium associated with diarrhoeal diseases and deep wound infections. Like many other microbial toxins, the protein changes in a multistep process from a completely water-soluble form to produce a transmembrane channel that destroys sensitive cells by breaking their permeability barriers. Here we describe the structure of proaerolysin determined by X-ray crystallography at 2.8 A resolution. The protoxin (M(r) 52,000) adopts a novel protein fold. Images of an aerolysin oligomer derived from electron microscopy have assisted in constructing a model of the membrane channel and have led to the proposal of a scheme to account for insertion of the protein into lipid bilayers to form ion channels.

==About this Structure==

3C0N is a ~~[[Single protein]]~~ structure of ~~sequence~~ from [http://en.wikipedia.org/wiki/Aeromonas_hydrophila Aeromonas hydrophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C0N OCA].

3C0N is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Aeromonas_hydrophila Aeromonas hydrophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C0N OCA].

==Reference==

Structure of the Aeromonas toxin proaerolysin in its water-soluble and membrane-channel states., Parker MW, Buckley JT, Postma JP, Tucker AD, Leonard K, Pattus F, Tsernoglou D, Nature. 1994 Jan 20;367(6460):~~292-5.~~ PMID:~~[http://www.ncbi.nlm.nih.gov~~/~~pubmed~~/~~7510043 7510043]~~

[[Category: Aeromonas hydrophila]]

~~[[Category: Single protein]]~~

[[Category: Burr, S E.]]

[[Category: Goot, G van der.]]

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[[Category: Pore-forming toxin]]

[[Category: Toxin]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sun May 4 21~~:15:~~53 2008~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 00:05:21 2009''

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-[[Image:3c0n.jpg|left|200px]]
+{{Seed}}
+[[Image:3c0n.png|left|200px]]
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@@ Line 9: / Line 10: @@
 {{STRUCTURE_3c0n|  PDB=3c0n  |  SCENE=  }}
-'''Crystal structure of the proaerolysin mutant Y221G at 2.2 A'''
+===Crystal structure of the proaerolysin mutant Y221G at 2.2 A===
-==Overview==
-Aerolysin is chiefly responsible for the pathogenicity of Aeromonas hydrophila, a bacterium associated with diarrhoeal diseases and deep wound infections. Like many other microbial toxins, the protein changes in a multistep process from a completely water-soluble form to produce a transmembrane channel that destroys sensitive cells by breaking their permeability barriers. Here we describe the structure of proaerolysin determined by X-ray crystallography at 2.8 A resolution. The protoxin (M(r) 52,000) adopts a novel protein fold. Images of an aerolysin oligomer derived from electron microscopy have assisted in constructing a model of the membrane channel and have led to the proposal of a scheme to account for insertion of the protein into lipid bilayers to form ion channels.
 ==About this Structure==
-C0N is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Aeromonas_hydrophila Aeromonas hydrophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C0N OCA].
+C0N is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Aeromonas_hydrophila Aeromonas hydrophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C0N OCA].
 ==Reference==
-Structure of the Aeromonas toxin proaerolysin in its water-soluble and membrane-channel states., Parker MW, Buckley JT, Postma JP, Tucker AD, Leonard K, Pattus F, Tsernoglou D, Nature. 1994 Jan 20;367(6460):292-5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7510043 7510043]
+<ref group="xtra">PMID:7510043</ref><ref group="xtra">PMID:2329576</ref><references group="xtra"/>
 [[Category: Aeromonas hydrophila]]
-[[Category: Single protein]]
 [[Category: Burr, S E.]]
 [[Category: Goot, G van der.]]
@@ Line 30: / Line 27: @@
 [[Category: Pore-forming toxin]]
 [[Category: Toxin]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 21:15:53 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 00:05:21 2009''