1as5: Difference between revisions

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[[Image:1as5.gif|left|200px]]<br /><applet load="1as5" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1as5.gif|left|200px]]<br /><applet load="1as5" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1as5" />

'''SOLUTION STRUCTURE OF CONOTOXIN Y-PIIIE FROM CONUS PURPURASCENS, NMR, 14 STRUCTURES'''<br />

==Overview==

The three-dimensional structure of conotoxin psi-PIIIE, a 24-amino acid, peptide from Conus purpurascens, has been solved using two-dimensional, (2D) 1H NMR spectroscopy. Conotoxin psi-PIIIE contains the same disulfide, bonding pattern as the mu-conotoxins, which target skeletal muscle sodium, channels, but has been shown to antagonize the acetylcholine gated cation, channel through a noncompetitive mechanism. Structural information was, obtained by the analysis of a series of 2D NOESY spectra as well as, measurement of coupling constants from 1D 1H and PE-COSY NMR experiments., Molecular modeling calculations included the use of the distance geometry, (DG) algorithm, simulated annealing techniques, and the restrained, molecular dynamics method. The resulting structures are considerably, similar to the previously published structures for the mu-conotoxins GIIIA, and GIIIB, despite the lack of sequence conservation between conotoxin, psi-PIIIE and the mu-conotoxins. The structure consists of a series of, tight turns, each turn occurring in the position analogous to those of, turns described in mu-GIIIA and mu-GIIIB. This suggests the disulfide, bonding pattern is able to largely direct the structure of the peptides, creating a stable structural motif which allows extensive sequence, substitution of non-cystine residues.

The three-dimensional structure of conotoxin psi-PIIIE, a 24-amino acid peptide from Conus purpurascens, has been solved using two-dimensional (2D) 1H NMR spectroscopy. Conotoxin psi-PIIIE contains the same disulfide bonding pattern as the mu-conotoxins, which target skeletal muscle sodium channels, but has been shown to antagonize the acetylcholine gated cation channel through a noncompetitive mechanism. Structural information was obtained by the analysis of a series of 2D NOESY spectra as well as measurement of coupling constants from 1D 1H and PE-COSY NMR experiments. Molecular modeling calculations included the use of the distance geometry (DG) algorithm, simulated annealing techniques, and the restrained molecular dynamics method. The resulting structures are considerably similar to the previously published structures for the mu-conotoxins GIIIA and GIIIB, despite the lack of sequence conservation between conotoxin psi-PIIIE and the mu-conotoxins. The structure consists of a series of tight turns, each turn occurring in the position analogous to those of turns described in mu-GIIIA and mu-GIIIB. This suggests the disulfide bonding pattern is able to largely direct the structure of the peptides, creating a stable structural motif which allows extensive sequence substitution of non-cystine residues.

==About this Structure==

1AS5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Conus_purpurascens Conus purpurascens] with NH2 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1AS5 OCA].

1AS5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Conus_purpurascens Conus purpurascens] with <scene name='pdbligand=NH2:'>NH2</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AS5 OCA].

==Reference==

Line 13:

[[Category: Conus purpurascens]]

[[Category: Single protein]]

[[Category: Foster, M.P.]]

[[Category: Foster, M P.]]

[[Category: Ireland, C.M.]]

[[Category: Ireland, C M.]]

[[Category: Mitchell, S.S.]]

[[Category: Mitchell, S S.]]

[[Category: Olivera, B.M.]]

[[Category: Olivera, B M.]]

[[Category: Shon, K.]]

[[Category: NH2]]

Line 23:

[[Category: neurotoxin]]

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@@ Line 1: / Line 1: @@
-[[Image:1as5.gif|left|200px]]<br /><applet load="1as5" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1as5.gif|left|200px]]<br /><applet load="1as5" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1as5" />
 '''SOLUTION STRUCTURE OF CONOTOXIN Y-PIIIE FROM CONUS PURPURASCENS, NMR, 14 STRUCTURES'''<br />
 ==Overview==
-The three-dimensional structure of conotoxin psi-PIIIE, a 24-amino acid, peptide from Conus purpurascens, has been solved using two-dimensional, (2D) 1H NMR spectroscopy. Conotoxin psi-PIIIE contains the same disulfide, bonding pattern as the mu-conotoxins, which target skeletal muscle sodium, channels, but has been shown to antagonize the acetylcholine gated cation, channel through a noncompetitive mechanism. Structural information was, obtained by the analysis of a series of 2D NOESY spectra as well as, measurement of coupling constants from 1D 1H and PE-COSY NMR experiments., Molecular modeling calculations included the use of the distance geometry, (DG) algorithm, simulated annealing techniques, and the restrained, molecular dynamics method. The resulting structures are considerably, similar to the previously published structures for the mu-conotoxins GIIIA, and GIIIB, despite the lack of sequence conservation between conotoxin, psi-PIIIE and the mu-conotoxins. The structure consists of a series of, tight turns, each turn occurring in the position analogous to those of, turns described in mu-GIIIA and mu-GIIIB. This suggests the disulfide, bonding pattern is able to largely direct the structure of the peptides, creating a stable structural motif which allows extensive sequence, substitution of non-cystine residues.
+The three-dimensional structure of conotoxin psi-PIIIE, a 24-amino acid peptide from Conus purpurascens, has been solved using two-dimensional (2D) 1H NMR spectroscopy. Conotoxin psi-PIIIE contains the same disulfide bonding pattern as the mu-conotoxins, which target skeletal muscle sodium channels, but has been shown to antagonize the acetylcholine gated cation channel through a noncompetitive mechanism. Structural information was obtained by the analysis of a series of 2D NOESY spectra as well as measurement of coupling constants from 1D 1H and PE-COSY NMR experiments. Molecular modeling calculations included the use of the distance geometry (DG) algorithm, simulated annealing techniques, and the restrained molecular dynamics method. The resulting structures are considerably similar to the previously published structures for the mu-conotoxins GIIIA and GIIIB, despite the lack of sequence conservation between conotoxin psi-PIIIE and the mu-conotoxins. The structure consists of a series of tight turns, each turn occurring in the position analogous to those of turns described in mu-GIIIA and mu-GIIIB. This suggests the disulfide bonding pattern is able to largely direct the structure of the peptides, creating a stable structural motif which allows extensive sequence substitution of non-cystine residues.
 ==About this Structure==
-AS5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Conus_purpurascens Conus purpurascens] with NH2 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1AS5 OCA].
+AS5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Conus_purpurascens Conus purpurascens] with <scene name='pdbligand=NH2:'>NH2</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AS5 OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Conus purpurascens]]
 [[Category: Single protein]]
-[[Category: Foster, M.P.]]
+[[Category: Foster, M P.]]
-[[Category: Ireland, C.M.]]
+[[Category: Ireland, C M.]]
-[[Category: Mitchell, S.S.]]
+[[Category: Mitchell, S S.]]
-[[Category: Olivera, B.M.]]
+[[Category: Olivera, B M.]]
 [[Category: Shon, K.]]
 [[Category: NH2]]
@@ Line 23: / Line 23: @@
 [[Category: neurotoxin]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 11:05:41 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:47:43 2008''