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| [[Image:3ygs.gif|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_3ygs| PDB=3ygs | SCENE= }} | | {{STRUCTURE_3ygs| PDB=3ygs | SCENE= }} |
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| '''APAF-1 CARD IN COMPLEX WITH PRODOMAIN OF PROCASPASE-9'''
| | ===APAF-1 CARD IN COMPLEX WITH PRODOMAIN OF PROCASPASE-9=== |
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| ==Overview==
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| Caspase-9-mediated apoptosis (programmed cell death) plays a central role in the development and homeostasis of all multicellular organisms. Mature caspase-9 is derived from its procaspase precursor as a result of recruitment by the activating factor Apaf-1. The crystal structures of the caspase-recruitment domain of Apaf-1 by itself and in complex with the prodomain of procaspase-9 have been determined at 1.6 and 2.5 A resolution, respectively. These structures and other evidence reveal that each molecule of Apaf-1 interacts with a molecule of procaspase-9 through two highly charged and complementary surfaces formed by non-conserved residues; these surfaces determine recognition specificity through networks of intermolecular hydrogen bonds and van der Waals interactions. Mutation of the important interface residues in procaspase-9 or Apaf-1 prevents or reduces activation of procaspase-9 in a cell-free system. Wild-type, but not mutant, prodomains of caspase-9 completely inhibit catalytic processing of procaspase-9. Furthermore, analysis of homologues from Caenorhabditis elegans indicates that recruitment of CED-3 by CED-4 is probably mediated by the same set of conserved structural motifs, with a corresponding change in the specificity-determining residues.
| | The line below this paragraph, {{ABSTRACT_PUBMED_10376594}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 10376594 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_10376594}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Caspase recruitment]] | | [[Category: Caspase recruitment]] |
| [[Category: Recognition complex]] | | [[Category: Recognition complex]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 22:15:41 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jul 3 13:13:30 2008'' |
