401d: Difference between revisions

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[[Image:401d.gif|left|200px]]
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[[Image:401d.png|left|200px]]


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{{STRUCTURE_401d|  PDB=401d  |  SCENE=  }}  
{{STRUCTURE_401d|  PDB=401d  |  SCENE=  }}  


'''STRUCTURE OF A DNA IN LOW SALT CONDITIONS D(GACCGCGGTC)'''
===STRUCTURE OF A DNA IN LOW SALT CONDITIONS D(GACCGCGGTC)===




==Overview==
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The X-ray crystal structure of the DNA decamer d(GACCGCGGTC), containing half the human papilloma virus E2 binding site, has been solved from two crystals grown at different ionic conditions (50 mM MgCl2and 50 mM spermine or 1.56 mM MgCl2and 1.56 mM spermine). Despite the variation in salt concentration, the two DNA structures are in a very similar, A-type DNA conformation, with helical axes curving towards the major groove. Although the salt concentrations do not effect the helical parameters or hydration to a large degree, there is a change in the overall helical curvature; 18 degrees and 31 degrees for the low and high salt structures, respectively. This curvature appears to be sequence specific and biologically relevant when compared with similar DNA structures, including the E2 binding site of a protein-DNA complex.
The line below this paragraph, {{ABSTRACT_PUBMED_9838004}}, adds the Publication Abstract to the page
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{{ABSTRACT_PUBMED_9838004}}


==About this Structure==
==About this Structure==
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[[Category: Luo, M.]]
[[Category: Luo, M.]]
[[Category: Dna double helix]]
[[Category: Dna double helix]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 22:16:03 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jul 3 13:14:26 2008''

Revision as of 10:14, 3 July 2008

File:401d.png

Template:STRUCTURE 401d

STRUCTURE OF A DNA IN LOW SALT CONDITIONS D(GACCGCGGTC)

Template:ABSTRACT PUBMED 9838004

About this Structure

Full crystallographic information is available from OCA.

Reference

X-ray crystal structures of half the human papilloma virus E2 binding site: d(GACCGCGGTC)., Finley JB, Luo M, Nucleic Acids Res. 1998 Dec 15;26(24):5719-27. PMID:9838004

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