437d: Difference between revisions

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{{STRUCTURE_437d|  PDB=437d  |  SCENE=  }}  
{{STRUCTURE_437d|  PDB=437d  |  SCENE=  }}  


'''CRYSTAL STRUCTURE OF AN RNA PSEUDOKNOT FROM BEET WESTERN YELLOW VIRUS INVOLVED IN RIBOSOMAL FRAMESHIFTING'''
===CRYSTAL STRUCTURE OF AN RNA PSEUDOKNOT FROM BEET WESTERN YELLOW VIRUS INVOLVED IN RIBOSOMAL FRAMESHIFTING===




==Overview==
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Many viruses regulate translation of polycistronic mRNA using a -1 ribosomal frameshift induced by an RNA pseudoknot. A pseudoknot has two stems that form a quasi-continuous helix and two connecting loops. A 1.6 A crystal structure of the beet western yellow virus (BWYV) pseudoknot reveals rotation and a bend at the junction of the two stems. A loop base is inserted in the major groove of one stem with quadruple-base interactions. The second loop forms a new minor-groove triplex motif with the other stem, involving 2'-OH and triple-base interactions, as well as sodium ion coordination. Overall, the number of hydrogen bonds stabilizing the tertiary interactions exceeds the number involved in Watson-Crick base pairs. This structure will aid mechanistic analyses of ribosomal frameshifting.
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==About this Structure==
==About this Structure==
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[[Category: Triplex]]
[[Category: Triplex]]
[[Category: Viral frameshifting]]
[[Category: Viral frameshifting]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 22:17:47 2008''
 
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Revision as of 10:19, 3 July 2008

File:437d.png

Template:STRUCTURE 437d

CRYSTAL STRUCTURE OF AN RNA PSEUDOKNOT FROM BEET WESTERN YELLOW VIRUS INVOLVED IN RIBOSOMAL FRAMESHIFTING

Template:ABSTRACT PUBMED 10074948

About this Structure

Full crystallographic information is available from OCA.

Reference

Minor groove RNA triplex in the crystal structure of a ribosomal frameshifting viral pseudoknot., Su L, Chen L, Egli M, Berger JM, Rich A, Nat Struct Biol. 1999 Mar;6(3):285-92. PMID:10074948

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