4rub: Difference between revisions

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[[Image:4rub.gif|left|200px]]
{{Seed}}
[[Image:4rub.png|left|200px]]


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{{STRUCTURE_4rub|  PDB=4rub  |  SCENE=  }}  
{{STRUCTURE_4rub|  PDB=4rub  |  SCENE=  }}  


'''A CRYSTAL FORM OF RIBULOSE-1,5-BISPHOSPHATE CARBOXYLASE(SLASH)OXYGENASE FROM NICOTIANA TABACUM IN THE ACTIVATED STATE'''
===A CRYSTAL FORM OF RIBULOSE-1,5-BISPHOSPHATE CARBOXYLASE(SLASH)OXYGENASE FROM NICOTIANA TABACUM IN THE ACTIVATED STATE===




==Overview==
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A new crystal form of ribulose-1,5-bisphosphate carboxylase/oxygenase (EC 4.1.1.39) from Nicotiana tabacum has been obtained at alkaline pH with polyethylene glycol 8000 in the presence of a non-ionic detergent, beta-octyl glucoside. The crystals are grown at room temperature by the hanging-drop vapor diffusion technique from a protein solution containing enzyme complexed with CO2, Mg2+, and the transition state analog 2-C-carboxy-D-arabinitol-1,5-bisphosphate. The crystals belong to the the space group P3(1)21 (or P3(2)21) with the cell parameters a = 204.6 A, and c = 117.4 A (1 A = 0.1 nm). The asymmetric unit contains half (L4S4: L, large subunit, 53,000 Mr; S, small subunit, 15,000 Mr) of a hexadecameric molecule (L8S8, 540,000 Mr). The crystals diffract to at least 2.6 A Bragg spacing and are suitable for X-ray structure determination.
The line below this paragraph, {{ABSTRACT_PUBMED_3681999}}, adds the Publication Abstract to the page
(as it appears on PubMed at http://www.pubmed.gov), where 3681999 is the PubMed ID number.
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{{ABSTRACT_PUBMED_3681999}}


==About this Structure==
==About this Structure==
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[[Category: Eisenberg, D.]]
[[Category: Eisenberg, D.]]
[[Category: Schreuder, H.]]
[[Category: Schreuder, H.]]
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