7gsp: Difference between revisions

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[[Image:7gsp.gif|left|200px]]
{{Seed}}
[[Image:7gsp.png|left|200px]]


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{{STRUCTURE_7gsp|  PDB=7gsp  |  SCENE=  }}  
{{STRUCTURE_7gsp|  PDB=7gsp  |  SCENE=  }}  


'''RIBONUCLEASE T1/2',3'-CGPS, NON-PRODUCTIVE'''
===RIBONUCLEASE T1/2',3'-CGPS, NON-PRODUCTIVE===




==Overview==
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Here we present a time-resolved crystallographic analysis of the hydrolysis of exo (Sp) guanosine 2',3'-cyclophosphorothioate by RNase T1. The use of a slow substrate and fast crystallization methods made it possible to perform the study with conventional data-collection techniques. The results support the idea that the hydrolysis reaction proceeds through a mechanism that is the inverse of the transesterification reaction. In addition, the structures provide an explanation for the differential behavior of RNase T1 towards exo- and endo-cyclic thiophosphates.
The line below this paragraph, {{ABSTRACT_PUBMED_9546218}}, adds the Publication Abstract to the page
(as it appears on PubMed at http://www.pubmed.gov), where 9546218 is the PubMed ID number.
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{{ABSTRACT_PUBMED_9546218}}


==About this Structure==
==About this Structure==
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[[Category: Hydrolase]]
[[Category: Hydrolase]]
[[Category: Ribonuclease]]
[[Category: Ribonuclease]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 22:45:01 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jul 3 14:33:58 2008''
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