9lyz: Difference between revisions

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[[Image:9lyz.gif|left|200px]]
{{Seed}}
[[Image:9lyz.png|left|200px]]


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{{STRUCTURE_9lyz|  PDB=9lyz  |  SCENE=  }}  
{{STRUCTURE_9lyz|  PDB=9lyz  |  SCENE=  }}  


'''X-RAY CRYSTALLOGRAPHY OF THE BINDING OF THE BACTERIAL CELL WALL TRISACCHARIDE NAM-NAG-NAM TO LYSOZYME'''
===X-RAY CRYSTALLOGRAPHY OF THE BINDING OF THE BACTERIAL CELL WALL TRISACCHARIDE NAM-NAG-NAM TO LYSOZYME===




==Overview==
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Hen egg white lysozyme was the first enzyme whose structure was determined by X-ray crystallography. The proposed mechanism based on this structure involves the distortion of the saccharide residue (2-acetamido-2-deoxy-D-muramic acid, NAM) in the natural substrate (an alternating beta (1 leads to 4) linked oligomer of 2-acetamido-2-deoxy-D-glucose (NAG) and NAM residues) bound to site D in the binding cleft. The importance of substrate distortion has prompted numerous enzymatic, chemical, theoretical, and physical studies, but there is little direct crystallographic evidence on the conformation of a NAM residue bound at site D. We now present the X-ray structure of the non-hydrolysed trisaccharide NAM-NAG-NAM bound in subsites B, C, D. Our interpretation of the 2.5-A resolution difference map does not involve distortion of this residue in site D. Comparison with the structure of the delta-lactone derived from tetra N-acetylchitotetraose (NAG)3NAL) bound to lysozyme suggests we may be looking at a Michaelis complex.
The line below this paragraph, {{ABSTRACT_PUBMED_514367}}, adds the Publication Abstract to the page
(as it appears on PubMed at http://www.pubmed.gov), where 514367 is the PubMed ID number.
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{{ABSTRACT_PUBMED_514367}}


==About this Structure==
==About this Structure==
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[[Category: James, M N.G.]]
[[Category: James, M N.G.]]
[[Category: Kelly, J A.]]
[[Category: Kelly, J A.]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 22:54:40 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Jul 11 12:52:38 2008''

Revision as of 09:52, 11 July 2008

File:9lyz.png

Template:STRUCTURE 9lyz

X-RAY CRYSTALLOGRAPHY OF THE BINDING OF THE BACTERIAL CELL WALL TRISACCHARIDE NAM-NAG-NAM TO LYSOZYME

Template:ABSTRACT PUBMED 514367

About this Structure

Full crystallographic information is available from OCA.

Reference

X-ray crystallography of the binding of the bacterial cell wall trisaccharide NAM-NAG-NAM to lysozyme., Kelly JA, Sielecki AR, Sykes BD, James MN, Phillips DC, Nature. 1979 Dec 20-27;282(5741):875-8. PMID:514367

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