3c0x: Difference between revisions

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[[Image:3c0x.jpg|left|200px]]
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[[Image:3c0x.png|left|200px]]


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{{STRUCTURE_3c0x|  PDB=3c0x  |  SCENE=  }}  
{{STRUCTURE_3c0x|  PDB=3c0x  |  SCENE=  }}  


'''I-SceI in complex with a top nicked DNA substrate'''
===I-SceI in complex with a top nicked DNA substrate===




==Overview==
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I-SceI is a homing endonuclease that specifically cleaves an 18-bp double-stranded DNA. I-SceI exhibits a strong preference for cleaving the bottom strand DNA. The published structure of I-SceI bound to an uncleaved DNA substrate provided a mechanism for bottom strand cleavage but not for top strand cleavage. To more fully elucidate the I-SceI catalytic mechanism, we determined the X-ray structures of I-SceI in complex with DNA substrates that are nicked in either the top or bottom strands. The structures resemble intermediates along the DNA cleavage reaction. In a structure containing a nick in the top strand, the spatial arrangement of metal ions is similar to that observed in the structure that contains uncleaved DNA, suggesting that cleavage of the bottom strand occurs by a common mechanism regardless of whether this strand is cleaved first or second. In the structure containing a nick in the bottom strand, a new metal binding site is present in the active site that cleaves the top strand. This new metal and a candidate nucleophilic water molecule are correctly positioned to cleave the top strand following bottom strand cleavage, providing a plausible mechanism for top strand cleavage.
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{{ABSTRACT_PUBMED_18424798}}


==About this Structure==
==About this Structure==
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[[Category: Mrna splicing]]
[[Category: Mrna splicing]]
[[Category: Nicked intermediate]]
[[Category: Nicked intermediate]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 9 10:10:05 2008''

Revision as of 07:10, 9 July 2008

File:3c0x.png

Template:STRUCTURE 3c0x

I-SceI in complex with a top nicked DNA substrate

Template:ABSTRACT PUBMED 18424798

About this Structure

3C0X is a Protein complex structure of sequences from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

Reference

Crystal structures of I-SceI complexed to nicked DNA substrates: snapshots of intermediates along the DNA cleavage reaction pathway., Moure CM, Gimble FS, Quiocho FA, Nucleic Acids Res. 2008 Apr 19;. PMID:18424798

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