1b5s: Difference between revisions
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New page: left|200px<br /><applet load="1b5s" size="450" color="white" frame="true" align="right" spinBox="true" caption="1b5s, resolution 4.4Å" /> '''DIHYDROLIPOYL TRANSAC... |
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[[Image:1b5s.jpg|left|200px]]<br /><applet load="1b5s" size=" | [[Image:1b5s.jpg|left|200px]]<br /><applet load="1b5s" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1b5s, resolution 4.4Å" /> | caption="1b5s, resolution 4.4Å" /> | ||
'''DIHYDROLIPOYL TRANSACETYLASE (E.C.2.3.1.12) CATALYTIC DOMAIN (RESIDUES 184-425) FROM BACILLUS STEAROTHERMOPHILUS'''<br /> | '''DIHYDROLIPOYL TRANSACETYLASE (E.C.2.3.1.12) CATALYTIC DOMAIN (RESIDUES 184-425) FROM BACILLUS STEAROTHERMOPHILUS'''<br /> | ||
==Overview== | ==Overview== | ||
The pyruvate dehydrogenase multienzyme complex (Mr of 5-10 million) is | The pyruvate dehydrogenase multienzyme complex (Mr of 5-10 million) is assembled around a structural core formed of multiple copies of dihydrolipoyl acetyltransferase (E2p), which exhibits the shape of either a cube or a dodecahedron, depending on the source. The crystal structures of the 60-meric dihydrolipoyl acyltransferase cores of Bacillus stearothermophilus and Enterococcus faecalis pyruvate dehydrogenase complexes were determined and revealed a remarkably hollow dodecahedron with an outer diameter of approximately 237 A, 12 large openings of approximately 52 A diameter across the fivefold axes, and an inner cavity with a diameter of approximately 118 A. Comparison of cubic and dodecahedral E2p assemblies shows that combining the principles of quasi-equivalence formulated by Caspar and Klug [Caspar, D. L. & Klug, A. (1962) Cold Spring Harbor Symp. Quant. Biol. 27, 1-4] with strict Euclidean geometric considerations results in predictions of the major features of the E2p dodecahedron matching the observed features almost exactly. | ||
==About this Structure== | ==About this Structure== | ||
1B5S is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]. Active as [http://en.wikipedia.org/wiki/Dihydrolipoyllysine-residue_acetyltransferase Dihydrolipoyllysine-residue acetyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.12 2.3.1.12] Full crystallographic information is available from [http:// | 1B5S is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]. Active as [http://en.wikipedia.org/wiki/Dihydrolipoyllysine-residue_acetyltransferase Dihydrolipoyllysine-residue acetyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.12 2.3.1.12] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B5S OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Aevarsson, A.]] | [[Category: Aevarsson, A.]] | ||
[[Category: Allen, M | [[Category: Allen, M D.]] | ||
[[Category: Hol, W | [[Category: Hol, W G.]] | ||
[[Category: Izard, T.]] | [[Category: Izard, T.]] | ||
[[Category: Kok, A | [[Category: Kok, A De.]] | ||
[[Category: Perham, R | [[Category: Perham, R N.]] | ||
[[Category: Westphal, A | [[Category: Westphal, A H.]] | ||
[[Category: dihydrolipoyl acetyltransferase]] | [[Category: dihydrolipoyl acetyltransferase]] | ||
[[Category: dihydrolipoyl transacetylase]] | [[Category: dihydrolipoyl transacetylase]] | ||
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[[Category: pyruvate dehydrogenase]] | [[Category: pyruvate dehydrogenase]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:51:50 2008'' | ||
