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[[Image:2jxs.jpg|left|200px]]
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[[Image:2jxs.png|left|200px]]


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{{STRUCTURE_2jxs|  PDB=2jxs  |  SCENE=  }}  
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'''NMR structure of RNA duplex containing single adenosine bulge'''
===NMR structure of RNA duplex containing single adenosine bulge===




==Overview==
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The RNA single bulge motif is an unpaired residue within a strand of several complementary base pairs. To gain insight into structural changes induced by the presence of the adenosine bulge on RNA duplex, the solution structures of RNA duplex containing a single adenine bulge (5'-GCAGAAGAGCG-3'/5'-CGCUCUCUGC-3') and a reference duplex with all Watson-Crick base pairs (5'-GCAGAGAGCG-3'/5'-CGCUCUCUGC-3') have been determined by NMR spectroscopy. The reference duplex structure is a regular right-handed helix with all of the attributes of an A-type helix. In the bulged duplex, single adenine bulge stacks into the helix, and the bulge region forms a well-defined structure. Both structures were analyzed by the use of calculated helical parameters. Distortions induced by the accommodation of unpaired residue into the helical structure propagate over the entire structure and are manifested as the reduced base pairs inclination and x-displacement. Intrahelical position of bulged adenine A5 is stabilized by efficient stacking with 5'-neighboring residues G4.
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{{ABSTRACT_PUBMED_18399645}}


==About this Structure==
==About this Structure==
2JXS is a [[Protein complex]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JXS OCA].  
2JXS is a [[Protein complex]] structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JXS OCA].  


==Reference==
==Reference==
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[[Category: Duplex]]
[[Category: Duplex]]
[[Category: Rna]]
[[Category: Rna]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu May 22 21:31:27 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 03:06:15 2008''

Revision as of 00:06, 28 July 2008

File:2jxs.png

Template:STRUCTURE 2jxs

NMR structure of RNA duplex containing single adenosine bulge

Template:ABSTRACT PUBMED 18399645

About this Structure

2JXS is a Protein complex structure. Full experimental information is available from OCA.

Reference

Bulged adenosine influence on the RNA duplex conformation in solution., Popenda L, Adamiak RW, Gdaniec Z, Biochemistry. 2008 May 6;47(18):5059-67. Epub 2008 Apr 10. PMID:18399645

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