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| {{STRUCTURE_3crf| PDB=3crf | SCENE= }} | | {{STRUCTURE_3crf| PDB=3crf | SCENE= }} |
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| '''Electron Microscopy model of the Saf Pilus- Type B'''
| | ===Electron Microscopy model of the Saf Pilus- Type B=== |
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| ==Overview==
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| Bacterial pili are important virulence factors involved in host cell attachment and/or biofilm formation, key steps in establishing and maintaining successful infection. Here we studied Salmonella atypical fimbriae (or Saf pili), formed by the conserved chaperone/usher pathway. In contrast to the well-established quaternary structure of typical/FGS-chaperone assembled, rod-shaped, chaperone/usher pili, little is known about the supramolecular organisation in atypical/FGL-chaperone assembled fimbriae. In our study, we have used negative stain electron microscopy and single-particle image analysis to determine the three-dimensional structure of the Salmonella typhimurium Saf pilus. Our results show atypical/FGL-chaperone assembled fimbriae are composed of highly flexible linear multi-subunit fibres that are formed by globular subunits connected to each other by short links giving a "beads on a string"-like appearance. Quantitative fitting of the atomic structure of the SafA pilus subunit into the electron density maps, in combination with linker modelling and energy minimisation, has enabled analysis of subunit arrangement and intersubunit interactions in the Saf pilus. Short intersubunit linker regions provide the molecular basis for flexibility of the Saf pilus by acting as molecular hinges allowing a large range of movement between consecutive subunits in the fibre.
| | The line below this paragraph, {{ABSTRACT_PUBMED_18448124}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 18448124 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_18448124}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Safa protein polymer]] | | [[Category: Safa protein polymer]] |
| [[Category: Type b saf pilus]] | | [[Category: Type b saf pilus]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu May 22 22:13:55 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 03:45:39 2008'' |
