2jrc: Difference between revisions

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[[Image:2jrc.jpg|left|200px]]
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{{STRUCTURE_2jrc|  PDB=2jrc  |  SCENE=  }}  
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'''Solution structure of Peptidyl-tRNA Hydrolase from Mycobacterium tuberculosis H37Rv.'''
===Solution structure of Peptidyl-tRNA Hydrolase from Mycobacterium tuberculosis H37Rv.===




==Overview==
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Eubacterial peptidyl-tRNA hydrolase is an essential enzyme that hydrolyzes peptidyl-tRNAs that are released into the cytoplasm because of premature termination of translation, expression of minigenes, and action of lincosamide and macrolide antibiotics. This averts the arrest of protein synthesis caused by depletion of free tRNA. Recently, we demonstrated that Mycobacterium tuberculosis peptidyl-tRNA hydrolase (MtPth) is present in the cytosol of mycobacterium and is capable of hydrolyzing peptidyl-tRNA. Here, we present the solution structure of MtPth, which is the first solution structure for this family of proteins. MtPth typically consists of seven-stranded mixed beta-sheet surrounded by six alpha-helices. The backbone dynamics for this enzyme were probed by measuring (15)N relaxation parameters and these were analyzed with model-free formalism and reduced spectral density mapping analysis. Overall, the protein molecule has tau(m) of 9.67+/-0.02 ns. The (15)N relaxation data analysis reveals that while majority of the protein backbone is rigid to motions, a short segment consisting of enzymatically critical residue H22, the loop-helix cover over the active site crevice, and the C-terminal helical hairpin exhibit motions on the milli-to microsecond timescale, all of which are linked to interaction with the substrate peptidyl-tRNA.
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{{ABSTRACT_PUBMED_18342886}}


==About this Structure==
==About this Structure==
2JRC is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JRC OCA].  
2JRC is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JRC OCA].  


==Reference==
==Reference==
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[[Category: Pth]]
[[Category: Pth]]
[[Category: Solution structure]]
[[Category: Solution structure]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed May 28 09:11:11 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 19:55:14 2008''

Revision as of 16:55, 27 July 2008

File:2jrc.png

Template:STRUCTURE 2jrc

Solution structure of Peptidyl-tRNA Hydrolase from Mycobacterium tuberculosis H37Rv.

Template:ABSTRACT PUBMED 18342886

About this Structure

2JRC is a Single protein structure of sequence from Mycobacterium tuberculosis. Full experimental information is available from OCA.

Reference

Solution structure and dynamics of peptidyl-tRNA hydrolase from Mycobacterium tuberculosis H37Rv., Pulavarti SV, Jain A, Pathak PP, Mahmood A, Arora A, J Mol Biol. 2008 Apr 18;378(1):165-77. Epub 2008 Feb 21. PMID:18342886

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