1bei: Difference between revisions

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[[Image:1bei.gif|left|200px]]<br /><applet load="1bei" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1bei.gif|left|200px]]<br /><applet load="1bei" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1bei" />

'''SHK-DNP22: A POTENT KV1.3-SPECIFIC IMMUNOSUPPRESSIVE POLYPEPTIDE, NMR, 20 STRUCTURES'''<br />

==Overview==

The voltage-gated potassium channel in T lymphocytes, Kv1.3, is an, important molecular target for immunosuppressive agents. A structurally, defined polypeptide, ShK, from the sea anemone Stichodactyla helianthus, inhibited Kv1.3 potently and also blocked Kv1.1, Kv1.4, and Kv1.6 at, subnanomolar concentrations. Using mutant cycle analysis in conjunction, with complementary mutagenesis of ShK and Kv1.3, and utilizing the, structure of ShK, we determined a likely docking configuration for this, peptide in the channel. Based upon this topological information, we, replaced the critical Lys22 in ShK with the positively charged, non-natural amino acid diaminopropionic acid (ShK-Dap22) and generated a, highly selective and potent blocker of the T-lymphocyte channel., ShK-Dap22, at subnanomolar concentrations, suppressed anti-CD3 induced, human T-lymphocyte [3H]thymidine incorporation in vitro. Toxicity with, this mutant peptide was low in a rodent model, with a median paralytic, dose of approximately 200 mg/kg body weight following intravenous, administration. The overall structure of ShK-Dap22 in solution, as, determined from NMR data, is similar to that of native ShK toxin, but, there are some differences in the residues involved in potassium channel, binding. Based on these results, we propose that ShK-Dap22 or a structural, analogue may have use as an immunosuppressant for the prevention of graft, rejection and for the treatment of autoimmune diseases.

The voltage-gated potassium channel in T lymphocytes, Kv1.3, is an important molecular target for immunosuppressive agents. A structurally defined polypeptide, ShK, from the sea anemone Stichodactyla helianthus inhibited Kv1.3 potently and also blocked Kv1.1, Kv1.4, and Kv1.6 at subnanomolar concentrations. Using mutant cycle analysis in conjunction with complementary mutagenesis of ShK and Kv1.3, and utilizing the structure of ShK, we determined a likely docking configuration for this peptide in the channel. Based upon this topological information, we replaced the critical Lys22 in ShK with the positively charged, non-natural amino acid diaminopropionic acid (ShK-Dap22) and generated a highly selective and potent blocker of the T-lymphocyte channel. ShK-Dap22, at subnanomolar concentrations, suppressed anti-CD3 induced human T-lymphocyte [3H]thymidine incorporation in vitro. Toxicity with this mutant peptide was low in a rodent model, with a median paralytic dose of approximately 200 mg/kg body weight following intravenous administration. The overall structure of ShK-Dap22 in solution, as determined from NMR data, is similar to that of native ShK toxin, but there are some differences in the residues involved in potassium channel binding. Based on these results, we propose that ShK-Dap22 or a structural analogue may have use as an immunosuppressant for the prevention of graft rejection and for the treatment of autoimmune diseases.

==About this Structure==

1BEI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Stichodactyla_helianthus Stichodactyla helianthus]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1BEI OCA].

1BEI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Stichodactyla_helianthus Stichodactyla helianthus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BEI OCA].

==Reference==

Line 13:

[[Category: Single protein]]

[[Category: Stichodactyla helianthus]]

[[Category: Cahalan, M.D.]]

[[Category: Cahalan, M D.]]

[[Category: Chandy, K.G.]]

[[Category: Chandy, K G.]]

[[Category: Christian, E.P.]]

[[Category: Christian, E P.]]

[[Category: Grissmer, S.]]

[[Category: Gutman, G.A.]]

[[Category: Gutman, G A.]]

[[Category: Kalman, K.]]

[[Category: Kem, W.R.]]

[[Category: Kem, W R.]]

[[Category: Lanigan, M.D.]]

[[Category: Lanigan, M D.]]

[[Category: Mahnir, V.]]

[[Category: Nguyen, A.]]

[[Category: Norton, R.S.]]

[[Category: Norton, R S.]]

[[Category: Paschetto, K.]]

[[Category: Pennington, M.W.]]

[[Category: Pennington, M W.]]

[[Category: Rauer, H.]]

[[Category: immunosuppressant neurotoxin]]

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[[Category: sea anemone]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Tue Nov 20~~ 11:35:~~12 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:54:28 2008''

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-[[Image:1bei.gif|left|200px]]<br /><applet load="1bei" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1bei.gif|left|200px]]<br /><applet load="1bei" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1bei" />
 '''SHK-DNP22: A POTENT KV1.3-SPECIFIC IMMUNOSUPPRESSIVE POLYPEPTIDE, NMR, 20 STRUCTURES'''<br />
 ==Overview==
-The voltage-gated potassium channel in T lymphocytes, Kv1.3, is an, important molecular target for immunosuppressive agents. A structurally, defined polypeptide, ShK, from the sea anemone Stichodactyla helianthus, inhibited Kv1.3 potently and also blocked Kv1.1, Kv1.4, and Kv1.6 at, subnanomolar concentrations. Using mutant cycle analysis in conjunction, with complementary mutagenesis of ShK and Kv1.3, and utilizing the, structure of ShK, we determined a likely docking configuration for this, peptide in the channel. Based upon this topological information, we, replaced the critical Lys22 in ShK with the positively charged, non-natural amino acid diaminopropionic acid (ShK-Dap22) and generated a, highly selective and potent blocker of the T-lymphocyte channel., ShK-Dap22, at subnanomolar concentrations, suppressed anti-CD3 induced, human T-lymphocyte [3H]thymidine incorporation in vitro. Toxicity with, this mutant peptide was low in a rodent model, with a median paralytic, dose of approximately 200 mg/kg body weight following intravenous, administration. The overall structure of ShK-Dap22 in solution, as, determined from NMR data, is similar to that of native ShK toxin, but, there are some differences in the residues involved in potassium channel, binding. Based on these results, we propose that ShK-Dap22 or a structural, analogue may have use as an immunosuppressant for the prevention of graft, rejection and for the treatment of autoimmune diseases.
+The voltage-gated potassium channel in T lymphocytes, Kv1.3, is an important molecular target for immunosuppressive agents. A structurally defined polypeptide, ShK, from the sea anemone Stichodactyla helianthus inhibited Kv1.3 potently and also blocked Kv1.1, Kv1.4, and Kv1.6 at subnanomolar concentrations. Using mutant cycle analysis in conjunction with complementary mutagenesis of ShK and Kv1.3, and utilizing the structure of ShK, we determined a likely docking configuration for this peptide in the channel. Based upon this topological information, we replaced the critical Lys22 in ShK with the positively charged, non-natural amino acid diaminopropionic acid (ShK-Dap22) and generated a highly selective and potent blocker of the T-lymphocyte channel. ShK-Dap22, at subnanomolar concentrations, suppressed anti-CD3 induced human T-lymphocyte [3H]thymidine incorporation in vitro. Toxicity with this mutant peptide was low in a rodent model, with a median paralytic dose of approximately 200 mg/kg body weight following intravenous administration. The overall structure of ShK-Dap22 in solution, as determined from NMR data, is similar to that of native ShK toxin, but there are some differences in the residues involved in potassium channel binding. Based on these results, we propose that ShK-Dap22 or a structural analogue may have use as an immunosuppressant for the prevention of graft rejection and for the treatment of autoimmune diseases.
 ==About this Structure==
-BEI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Stichodactyla_helianthus Stichodactyla helianthus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1BEI OCA].
+BEI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Stichodactyla_helianthus Stichodactyla helianthus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BEI OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Single protein]]
 [[Category: Stichodactyla helianthus]]
-[[Category: Cahalan, M.D.]]
+[[Category: Cahalan, M D.]]
-[[Category: Chandy, K.G.]]
+[[Category: Chandy, K G.]]
-[[Category: Christian, E.P.]]
+[[Category: Christian, E P.]]
 [[Category: Grissmer, S.]]
-[[Category: Gutman, G.A.]]
+[[Category: Gutman, G A.]]
 [[Category: Kalman, K.]]
-[[Category: Kem, W.R.]]
+[[Category: Kem, W R.]]
-[[Category: Lanigan, M.D.]]
+[[Category: Lanigan, M D.]]
 [[Category: Mahnir, V.]]
 [[Category: Nguyen, A.]]
-[[Category: Norton, R.S.]]
+[[Category: Norton, R S.]]
 [[Category: Paschetto, K.]]
-[[Category: Pennington, M.W.]]
+[[Category: Pennington, M W.]]
 [[Category: Rauer, H.]]
 [[Category: immunosuppressant neurotoxin]]
@@ Line 31: / Line 31: @@
 [[Category: sea anemone]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 11:35:12 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:54:28 2008''