1bg7: Difference between revisions
New page: left|200px<br /><applet load="1bg7" size="450" color="white" frame="true" align="right" spinBox="true" caption="1bg7, resolution 1.85Å" /> '''LOCALIZED UNFOLDING ... |
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[[Image:1bg7.gif|left|200px]]<br /><applet load="1bg7" size=" | [[Image:1bg7.gif|left|200px]]<br /><applet load="1bg7" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1bg7, resolution 1.85Å" /> | caption="1bg7, resolution 1.85Å" /> | ||
'''LOCALIZED UNFOLDING AT THE JUNCTION OF THREE FERRITIN SUBUNITS. A MECHANISM FOR IRON RELEASE?'''<br /> | '''LOCALIZED UNFOLDING AT THE JUNCTION OF THREE FERRITIN SUBUNITS. A MECHANISM FOR IRON RELEASE?'''<br /> | ||
==Overview== | ==Overview== | ||
How and where iron exits from ferritin for cellular use is unknown. | How and where iron exits from ferritin for cellular use is unknown. Twenty-four protein subunits create a cavity in ferritin where iron is concentrated >10(11)-fold as a mineral. Proline substitution for conserved leucine 134 (L134P) allowed normal assembly but increased iron exit rates. X-ray crystallography of H-L134P ferritin revealed localized unfolding at the 3-fold axis, also iron entry sites, consistent with shared use sites for iron exit and entry. The junction of three ferritin subunits appears to be a dynamic aperture with a "shutter" that cytoplasmic factors might open or close to regulate iron release in vivo. | ||
==About this Structure== | ==About this Structure== | ||
1BG7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rana_catesbeiana Rana catesbeiana] with CA as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | 1BG7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rana_catesbeiana Rana catesbeiana] with <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BG7 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Rana catesbeiana]] | [[Category: Rana catesbeiana]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Allewell, N | [[Category: Allewell, N M.]] | ||
[[Category: Ha, Y.]] | [[Category: Ha, Y.]] | ||
[[Category: Shi, D.]] | [[Category: Shi, D.]] | ||
[[Category: Takagi, H.]] | [[Category: Takagi, H.]] | ||
[[Category: Theil, E | [[Category: Theil, E C.]] | ||
[[Category: CA]] | [[Category: CA]] | ||
[[Category: ferritin]] | [[Category: ferritin]] | ||
[[Category: iron storage]] | [[Category: iron storage]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:54:56 2008'' | ||
Revision as of 09:54, 21 February 2008
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LOCALIZED UNFOLDING AT THE JUNCTION OF THREE FERRITIN SUBUNITS. A MECHANISM FOR IRON RELEASE?
Overview
How and where iron exits from ferritin for cellular use is unknown. Twenty-four protein subunits create a cavity in ferritin where iron is concentrated >10(11)-fold as a mineral. Proline substitution for conserved leucine 134 (L134P) allowed normal assembly but increased iron exit rates. X-ray crystallography of H-L134P ferritin revealed localized unfolding at the 3-fold axis, also iron entry sites, consistent with shared use sites for iron exit and entry. The junction of three ferritin subunits appears to be a dynamic aperture with a "shutter" that cytoplasmic factors might open or close to regulate iron release in vivo.
About this Structure
1BG7 is a Single protein structure of sequence from Rana catesbeiana with CA as ligand. Full crystallographic information is available from OCA.
Reference
Localized unfolding at the junction of three ferritin subunits. A mechanism for iron release?, Takagi H, Shi D, Ha Y, Allewell NM, Theil EC, J Biol Chem. 1998 Jul 24;273(30):18685-8. PMID:9668036
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