1bjf: Difference between revisions

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[[Image:1bjf.jpg|left|200px]]<br /><applet load="1bjf" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1bjf.jpg|left|200px]]<br /><applet load="1bjf" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1bjf, resolution 2.4Å" />

'''CRYSTAL STRUCTURE OF RECOMBINANT BOVINE NEUROCALCIN DELTA AT 2.4 ANGSTROMS'''<br />

==Overview==

The crystal structure of calcium-bound unmyristoylated bovine neurocalcin, from Escherichia coli has been determined at 2.4 A resolution. The, three-dimensional structure reveals a highly compact structure consisting, of: (i) two pairs of calcium-binding EF-hands (EF1-EF2 and EF3-EF4); (ii), a calcium ion bound at EF2, EF3 and EF4 sites; and (iii) an EF1-hand that, is disabled from calcium-binding due to a Cys-Pro sequence in the, Ca2+-binding loop. The crystal structure of neurocalcin resembles, photoreceptor recoverin in overall topology, however its EF2- and, EF4-hands differ. Recently, neurocalcin in the calcium-bound state has, been shown to stimulate mammalian rod outer segment membrane guanylate, cyclase. A possible site for cyclase activity based on the, three-dimensional structure is discussed.

The crystal structure of calcium-bound unmyristoylated bovine neurocalcin from Escherichia coli has been determined at 2.4 A resolution. The three-dimensional structure reveals a highly compact structure consisting of: (i) two pairs of calcium-binding EF-hands (EF1-EF2 and EF3-EF4); (ii) a calcium ion bound at EF2, EF3 and EF4 sites; and (iii) an EF1-hand that is disabled from calcium-binding due to a Cys-Pro sequence in the Ca2+-binding loop. The crystal structure of neurocalcin resembles photoreceptor recoverin in overall topology, however its EF2- and EF4-hands differ. Recently, neurocalcin in the calcium-bound state has been shown to stimulate mammalian rod outer segment membrane guanylate cyclase. A possible site for cyclase activity based on the three-dimensional structure is discussed.

==About this Structure==

1BJF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with CA as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1BJF OCA].

1BJF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BJF OCA].

==Reference==

Line 13:

[[Category: Escherichia coli]]

[[Category: Single protein]]

[[Category: Kumar, V.D.]]

[[Category: Kumar, V D.]]

[[Category: Vijay-Kumar, S.]]

[[Category: CA]]

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[[Category: neuronal specific guanylate cyclase activator]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Tue Nov 20~~ 11:41:~~47 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:55:56 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1bjf.jpg|left|200px]]<br /><applet load="1bjf" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1bjf.jpg|left|200px]]<br /><applet load="1bjf" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1bjf, resolution 2.4&Aring;" />
 '''CRYSTAL STRUCTURE OF RECOMBINANT BOVINE NEUROCALCIN DELTA AT 2.4 ANGSTROMS'''<br />
 ==Overview==
-The crystal structure of calcium-bound unmyristoylated bovine neurocalcin, from Escherichia coli has been determined at 2.4 A resolution. The, three-dimensional structure reveals a highly compact structure consisting, of: (i) two pairs of calcium-binding EF-hands (EF1-EF2 and EF3-EF4); (ii), a calcium ion bound at EF2, EF3 and EF4 sites; and (iii) an EF1-hand that, is disabled from calcium-binding due to a Cys-Pro sequence in the, Ca2+-binding loop. The crystal structure of neurocalcin resembles, photoreceptor recoverin in overall topology, however its EF2- and, EF4-hands differ. Recently, neurocalcin in the calcium-bound state has, been shown to stimulate mammalian rod outer segment membrane guanylate, cyclase. A possible site for cyclase activity based on the, three-dimensional structure is discussed.
+The crystal structure of calcium-bound unmyristoylated bovine neurocalcin from Escherichia coli has been determined at 2.4 A resolution. The three-dimensional structure reveals a highly compact structure consisting of: (i) two pairs of calcium-binding EF-hands (EF1-EF2 and EF3-EF4); (ii) a calcium ion bound at EF2, EF3 and EF4 sites; and (iii) an EF1-hand that is disabled from calcium-binding due to a Cys-Pro sequence in the Ca2+-binding loop. The crystal structure of neurocalcin resembles photoreceptor recoverin in overall topology, however its EF2- and EF4-hands differ. Recently, neurocalcin in the calcium-bound state has been shown to stimulate mammalian rod outer segment membrane guanylate cyclase. A possible site for cyclase activity based on the three-dimensional structure is discussed.
 ==About this Structure==
-BJF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with CA as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1BJF OCA].
+BJF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BJF OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Escherichia coli]]
 [[Category: Single protein]]
-[[Category: Kumar, V.D.]]
+[[Category: Kumar, V D.]]
 [[Category: Vijay-Kumar, S.]]
 [[Category: CA]]
@@ Line 20: / Line 20: @@
 [[Category: neuronal specific guanylate cyclase activator]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 11:41:47 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:55:56 2008''