1bob: Difference between revisions
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New page: left|200px<br /><applet load="1bob" size="450" color="white" frame="true" align="right" spinBox="true" caption="1bob, resolution 2.3Å" /> '''HISTONE ACETYLTRANSFE... |
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[[Image:1bob.jpg|left|200px]]<br /><applet load="1bob" size=" | [[Image:1bob.jpg|left|200px]]<br /><applet load="1bob" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1bob, resolution 2.3Å" /> | caption="1bob, resolution 2.3Å" /> | ||
'''HISTONE ACETYLTRANSFERASE HAT1 FROM SACCHAROMYCES CEREVISIAE IN COMPLEX WITH ACETYL COENZYME A'''<br /> | '''HISTONE ACETYLTRANSFERASE HAT1 FROM SACCHAROMYCES CEREVISIAE IN COMPLEX WITH ACETYL COENZYME A'''<br /> | ||
==Overview== | ==Overview== | ||
We have solved the crystal structure of the yeast histone | We have solved the crystal structure of the yeast histone acetyltransferase Hat1-acetyl coenzyme A (AcCoA) complex at 2.3 A resolution. Hat1 has an elongated, curved structure, and the AcCoA molecule is bound in a cleft on the concave surface of the protein, marking the active site of the enzyme. A channel of variable width and depth that runs across the protein is probably the binding site for the histone substrate. A model for histone H4 binding by Hat1 is discussed in terms of possible sources of specific lysine recognition by the enzyme. The structure of Hat1 provides a model for the structures of the catalytic domains of a protein superfamily that includes other histone acetyltransferases such as Gcn5 and CBP. | ||
==About this Structure== | ==About this Structure== | ||
1BOB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with CA and ACO as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Histone_acetyltransferase Histone acetyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.48 2.3.1.48] Full crystallographic information is available from [http:// | 1BOB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=ACO:'>ACO</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Histone_acetyltransferase Histone acetyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.48 2.3.1.48] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BOB OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Dutnall, R | [[Category: Dutnall, R N.]] | ||
[[Category: Ramakrishnan, V.]] | [[Category: Ramakrishnan, V.]] | ||
[[Category: Sternglanz, R.]] | [[Category: Sternglanz, R.]] | ||
[[Category: Tafrov, S | [[Category: Tafrov, S T.]] | ||
[[Category: ACO]] | [[Category: ACO]] | ||
[[Category: CA]] | [[Category: CA]] | ||
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[[Category: histone modification]] | [[Category: histone modification]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:57:25 2008'' | ||
