1bpt: Difference between revisions

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[[Image:1bpt.gif|left|200px]]<br /><applet load="1bpt" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1bpt.gif|left|200px]]<br /><applet load="1bpt" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1bpt, resolution 2.0Å" />

'''CREVICE-FORMING MUTANTS OF BPTI: CRYSTAL STRUCTURES OF F22A, Y23A, N43G, AND F45A'''<br />

==Overview==

Crystal structures of four mutants of bovine pancreatic trypsin inhibitor, (F22A, Y23A, N43G, and F45A), engineered to alter their stability, properties, have been determined. The mutated residues, which are highly, conserved among Kunitz-type inhibitors, are located in the rigid core of, the molecule. Replacement of the partially buried bulky residues of the, wild-type protein with smaller residues resulted in crevices open to the, exterior of the molecule. The overall three-dimensional structure of these, mutants is very similar to that of the wild-type protein and only small, rearrangements are observed among the atoms lining the crevices.

Crystal structures of four mutants of bovine pancreatic trypsin inhibitor (F22A, Y23A, N43G, and F45A), engineered to alter their stability properties, have been determined. The mutated residues, which are highly conserved among Kunitz-type inhibitors, are located in the rigid core of the molecule. Replacement of the partially buried bulky residues of the wild-type protein with smaller residues resulted in crevices open to the exterior of the molecule. The overall three-dimensional structure of these mutants is very similar to that of the wild-type protein and only small rearrangements are observed among the atoms lining the crevices.

==About this Structure==

1BPT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with PO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1BPT OCA].

1BPT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with <scene name='pdbligand=PO4:'>PO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BPT OCA].

==Reference==

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[[Category: proteinase inhibitor (trypsin)]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Tue Nov 20~~ 11:49:~~21 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:57:48 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1bpt.gif|left|200px]]<br /><applet load="1bpt" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1bpt.gif|left|200px]]<br /><applet load="1bpt" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1bpt, resolution 2.0&Aring;" />
 '''CREVICE-FORMING MUTANTS OF BPTI: CRYSTAL STRUCTURES OF F22A, Y23A, N43G, AND F45A'''<br />
 ==Overview==
-Crystal structures of four mutants of bovine pancreatic trypsin inhibitor, (F22A, Y23A, N43G, and F45A), engineered to alter their stability, properties, have been determined. The mutated residues, which are highly, conserved among Kunitz-type inhibitors, are located in the rigid core of, the molecule. Replacement of the partially buried bulky residues of the, wild-type protein with smaller residues resulted in crevices open to the, exterior of the molecule. The overall three-dimensional structure of these, mutants is very similar to that of the wild-type protein and only small, rearrangements are observed among the atoms lining the crevices.
+Crystal structures of four mutants of bovine pancreatic trypsin inhibitor (F22A, Y23A, N43G, and F45A), engineered to alter their stability properties, have been determined. The mutated residues, which are highly conserved among Kunitz-type inhibitors, are located in the rigid core of the molecule. Replacement of the partially buried bulky residues of the wild-type protein with smaller residues resulted in crevices open to the exterior of the molecule. The overall three-dimensional structure of these mutants is very similar to that of the wild-type protein and only small rearrangements are observed among the atoms lining the crevices.
 ==About this Structure==
-BPT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with PO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1BPT OCA].
+BPT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with <scene name='pdbligand=PO4:'>PO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BPT OCA].
 ==Reference==
@@ Line 21: / Line 21: @@
 [[Category: proteinase inhibitor (trypsin)]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 11:49:21 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:57:48 2008''