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| {{STRUCTURE_2zea| PDB=2zea | SCENE= }} | | {{STRUCTURE_2zea| PDB=2zea | SCENE= }} |
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| '''Crystal structure of Alcaligenes faecalis D-3-hydroxybutyrate dehydrogenase in complex with NAD+ and acetate'''
| | ===Crystal structure of Alcaligenes faecalis D-3-hydroxybutyrate dehydrogenase in complex with NAD+ and acetate=== |
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| ==Overview==
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| D-3-Hydroxybutyrate dehydrogenase, which catalyzes the reversible reaction between D-3-hydroxybutyrate and acetoacetate, has been classified into the short-chain dehydrogenase/reductase family and is a useful marker in the assay of diabetes mellitus and/or ketoacidosis. The enzyme from Alcaligenes faecalis was crystallized in the apo form and in the holo form with acetate as a substrate analogue. The crystal structures of both forms were determined at 2.2 angstroms resolution. The enzyme is a tetramer composed of four subunits assembled with noncrystallographic 222 point symmetry. Each subunit has two domains. The principal domain adopts the Rossmann fold essential for nucleotide binding, which is a common feature of the SDR family. NAD+ is bound in a large cleft in the domain. The pyrophosphate group of NAD+ is covered by the small additional domain, which is supported by two extended arms allowing domain movement. In the catalytic site, a water molecule is trapped by the catalytic Tyr155 and Ser142 residues in the vicinity of the bound NAD+ and acetate. The substrate analogue acetate is bound above the nicotinamide plane. A substrate (D-3-hydroxybutylate) bound model can reasonably be constructed by adding two C atoms into the void space between the water O atom and the methyl group of the acetate, suggesting a substrate-bound state before enzymatic reaction occurs. Based on these structural features, a reaction mechanism has been proposed.
| | The line below this paragraph, {{ABSTRACT_PUBMED_18453685}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 18453685 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_18453685}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Nad dependent enzyme]] | | [[Category: Nad dependent enzyme]] |
| [[Category: Oxidoreductase]] | | [[Category: Oxidoreductase]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 4 09:53:51 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 18:55:37 2008'' |
