1c5f: Difference between revisions

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[[Image:1c5f.gif|left|200px]]<br /><applet load="1c5f" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1c5f.gif|left|200px]]<br /><applet load="1c5f" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1c5f, resolution 2.47Å" />

'''CRYSTAL STRUCTURE OF THE CYCLOPHILIN-LIKE DOMAIN FROM BRUGIA MALAYI COMPLEXED WITH CYCLOSPORIN A'''<br />

==Overview==

The resistance of the human parasite Brugia malayi to the antiparasitic, activity of cyclosporin A (CsA) may arise from the presence of, cyclophilins with relatively low affinity for the drug. The structure of, the complex of B. malayi cyclophilin (BmCYP-1) and CsA, with eight, independent copies in the asymmetric unit, has been determined at a, resolution of 2.7 A. The low affinity of BmCYP-1 for CsA arises from, incomplete preorganization of the binding site so that the formation of a, hydrogen bond between His132 of BmCYP-1 and N-methylleucine 9 of CsA is, associated with a shift in the backbone of approximately 1 A in this, region.

The resistance of the human parasite Brugia malayi to the antiparasitic activity of cyclosporin A (CsA) may arise from the presence of cyclophilins with relatively low affinity for the drug. The structure of the complex of B. malayi cyclophilin (BmCYP-1) and CsA, with eight independent copies in the asymmetric unit, has been determined at a resolution of 2.7 A. The low affinity of BmCYP-1 for CsA arises from incomplete preorganization of the binding site so that the formation of a hydrogen bond between His132 of BmCYP-1 and N-methylleucine 9 of CsA is associated with a shift in the backbone of approximately 1 A in this region.

==About this Structure==

1C5F is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Brugia_malayi Brugia malayi]. This structure ~~superseeds~~ the now removed PDB entry 1QTL. Active as [http://en.wikipedia.org/wiki/Peptidylprolyl_isomerase Peptidylprolyl isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.2.1.8 5.2.1.8] Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1C5F OCA].

1C5F is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Brugia_malayi Brugia malayi]. This structure supersedes the now removed PDB entry 1QTL. Active as [http://en.wikipedia.org/wiki/Peptidylprolyl_isomerase Peptidylprolyl isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.2.1.8 5.2.1.8] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1C5F OCA].

==Reference==

Line 14:

[[Category: Peptidylprolyl isomerase]]

[[Category: Single protein]]

[[Category: Carlow, C.K.S.]]

[[Category: Carlow, C K.S.]]

[[Category: Ellis, P.J.]]

[[Category: Ellis, P J.]]

[[Category: Kuhn, P.]]

[[Category: Ma, D.]]

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[[Category: peptidylprolyl isomerase]]

''Page seeded by [http://~~ispc~~.weizmann.ac.il/oca OCA ] on ~~Tue Nov 20~~ 12:10:~~23 2007~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:02:33 2008''

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@@ Line 1: / Line 1: @@
-[[Image:1c5f.gif|left|200px]]<br /><applet load="1c5f" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1c5f.gif|left|200px]]<br /><applet load="1c5f" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1c5f, resolution 2.47&Aring;" />
 '''CRYSTAL STRUCTURE OF THE CYCLOPHILIN-LIKE DOMAIN FROM BRUGIA MALAYI COMPLEXED WITH CYCLOSPORIN A'''<br />
 ==Overview==
-The resistance of the human parasite Brugia malayi to the antiparasitic, activity of cyclosporin A (CsA) may arise from the presence of, cyclophilins with relatively low affinity for the drug. The structure of, the complex of B. malayi cyclophilin (BmCYP-1) and CsA, with eight, independent copies in the asymmetric unit, has been determined at a, resolution of 2.7 A. The low affinity of BmCYP-1 for CsA arises from, incomplete preorganization of the binding site so that the formation of a, hydrogen bond between His132 of BmCYP-1 and N-methylleucine 9 of CsA is, associated with a shift in the backbone of approximately 1 A in this, region.
+The resistance of the human parasite Brugia malayi to the antiparasitic activity of cyclosporin A (CsA) may arise from the presence of cyclophilins with relatively low affinity for the drug. The structure of the complex of B. malayi cyclophilin (BmCYP-1) and CsA, with eight independent copies in the asymmetric unit, has been determined at a resolution of 2.7 A. The low affinity of BmCYP-1 for CsA arises from incomplete preorganization of the binding site so that the formation of a hydrogen bond between His132 of BmCYP-1 and N-methylleucine 9 of CsA is associated with a shift in the backbone of approximately 1 A in this region.
 ==About this Structure==
-C5F is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Brugia_malayi Brugia malayi]. This structure superseeds the now removed PDB entry 1QTL. Active as [http://en.wikipedia.org/wiki/Peptidylprolyl_isomerase Peptidylprolyl isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.2.1.8 5.2.1.8] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1C5F OCA].
+C5F is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Brugia_malayi Brugia malayi]. This structure supersedes the now removed PDB entry 1QTL. Active as [http://en.wikipedia.org/wiki/Peptidylprolyl_isomerase Peptidylprolyl isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.2.1.8 5.2.1.8] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1C5F OCA].
 ==Reference==
@@ Line 14: / Line 14: @@
 [[Category: Peptidylprolyl isomerase]]
 [[Category: Single protein]]
-[[Category: Carlow, C.K.S.]]
+[[Category: Carlow, C K.S.]]
-[[Category: Ellis, P.J.]]
+[[Category: Ellis, P J.]]
 [[Category: Kuhn, P.]]
 [[Category: Ma, D.]]
@@ Line 24: / Line 24: @@
 [[Category: peptidylprolyl isomerase]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 12:10:23 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:02:33 2008''