3c68: Difference between revisions

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~~'''Unreleased structure'''~~

[[Image:3c68.jpg|left|200px]]

The ~~entry~~ 3c68 ~~is ON HOLD~~ ~~until Paper Publication~~

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~~Authors: Kurakata, Y., Uechi, A., Yoshida, H., Kamitori, S., Sakano, Y., Nishikawa, A., Tonozuka, T.~~

'''Escherichia coli K12 YgjK in a complexed with mannose'''

~~Description: Escherichia coli K12 YgjK in a complexed with mannose~~

==Overview==

Processing alpha-glucosidase I, which is classified into glycosyl hydrolase (GH) family 63, hydrolyzes an oligosaccharide precursor of eukaryotic N-linked glycoproteins. Recently, many bacteria have been reported to possess genes for proteins that are homologous to the GH family 63 glucosidases. In this paper, Escherichia coli K12 YgjK protein, a member of GH family 63, was overexpressed, purified and crystallized using the vapour-diffusion method. Diffraction data were collected to 1.8 A resolution and the crystal was found to belong to the monoclinic space group P2(1), with unit-cell parameters a = 88.5, b = 137.1, c = 60.9 A, beta = 98.1 degrees. The V(M) value was determined to be 2.1 A(3) Da(-1), which corresponds to the presence of two protein molecules in the asymmetric unit.

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 11 09:30:02 2008''

==About this Structure==

3C68 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C68 OCA].

==Reference==

Crystallization and preliminary X-ray analysis of Escherichia coli K12 YgjK protein, a member of glycosyl hydrolase family 63., Tonozuka T, Uechi A, Mizuno M, Ichikawa K, Nishikawa A, Sakano Y, Acta Crystallogr D Biol Crystallogr. 2004 Jul;60(Pt 7):1284-5. Epub 2004, Jun 22. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15213393 15213393]

[[Category: Escherichia coli]]

[[Category: Single protein]]

[[Category: Kamitori, S.]]

[[Category: Kurakata, Y.]]

[[Category: Nishikawa, A.]]

[[Category: Sakano, Y.]]

[[Category: Tonozuka, T.]]

[[Category: Uechi, A.]]

[[Category: Yoshida, H.]]

[[Category: Alpha/alpha barrel]]

[[Category: Gh63]]

[[Category: Hydrolase]]

[[Category: Processing alpha-glucosidase]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 11 10:48:33 2008''

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@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
+[[Image:3c68.jpg|left|200px]]
-The entry 3c68 is ON HOLD  until Paper Publication
+<!--
+The line below this paragraph, containing "STRUCTURE_3c68", creates the "Structure Box" on the page.
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+or leave the SCENE parameter empty for the default display.
+-->
+{{STRUCTURE_3c68|  PDB=3c68  |  SCENE=  }}
-Authors: Kurakata, Y., Uechi, A., Yoshida, H., Kamitori, S., Sakano, Y., Nishikawa, A., Tonozuka, T.
+'''Escherichia coli K12 YgjK in a complexed with mannose'''
-Description: Escherichia coli K12 YgjK in a complexed with mannose
+==Overview==
+Processing alpha-glucosidase I, which is classified into glycosyl hydrolase (GH) family 63, hydrolyzes an oligosaccharide precursor of eukaryotic N-linked glycoproteins. Recently, many bacteria have been reported to possess genes for proteins that are homologous to the GH family 63 glucosidases. In this paper, Escherichia coli K12 YgjK protein, a member of GH family 63, was overexpressed, purified and crystallized using the vapour-diffusion method. Diffraction data were collected to 1.8 A resolution and the crystal was found to belong to the monoclinic space group P2(1), with unit-cell parameters a = 88.5, b = 137.1, c = 60.9 A, beta = 98.1 degrees. The V(M) value was determined to be 2.1 A(3) Da(-1), which corresponds to the presence of two protein molecules in the asymmetric unit.
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 11 09:30:02 2008''
+==About this Structure==
+C68 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C68 OCA].
+==Reference==
+Crystallization and preliminary X-ray analysis of Escherichia coli K12 YgjK protein, a member of glycosyl hydrolase family 63., Tonozuka T, Uechi A, Mizuno M, Ichikawa K, Nishikawa A, Sakano Y, Acta Crystallogr D Biol Crystallogr. 2004 Jul;60(Pt 7):1284-5. Epub 2004, Jun 22. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15213393 15213393]
+[[Category: Escherichia coli]]
+[[Category: Single protein]]
+[[Category: Kamitori, S.]]
+[[Category: Kurakata, Y.]]
+[[Category: Nishikawa, A.]]
+[[Category: Sakano, Y.]]
+[[Category: Tonozuka, T.]]
+[[Category: Uechi, A.]]
+[[Category: Yoshida, H.]]
+[[Category: Alpha/alpha barrel]]
+[[Category: Gh63]]
+[[Category: Hydrolase]]
+[[Category: Processing alpha-glucosidase]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 11 10:48:33 2008''