3d8d: Difference between revisions

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[[Image:3d8d.jpg|left|200px]]
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[[Image:3d8d.png|left|200px]]


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{{STRUCTURE_3d8d|  PDB=3d8d  |  SCENE=  }}  
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'''Crystal structure of the human Fe65-PTB1 domain'''
===Crystal structure of the human Fe65-PTB1 domain===




==Overview==
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Fe65 is a three-domain neuronal adaptor protein involved in brain development and amyloid precursor protein (APP) signalling. The phosphotyrosine-binding domain 1 (PTB1) of human Fe65 has been cloned, overexpressed, purified and crystallized using the hanging-drop vapour-diffusion method. Native crystals belong to the space group R3 and diffract to 2.6 A resolution. This crystal form suffered from high thermal B factors and pseudo-symmetry, resulting in a bisection of the c axis. Co-crystallization with a mercury compound under similar conditions induced an orthorhombic crystal form in the space group P2(1)2(1)2(1) diffracting to 2.2 A resolution. SAD phases have been computed to the diffraction limit at the wavelength of maximum absorption (L(III) edge).
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{{ABSTRACT_PUBMED_18550529}}


==About this Structure==
==About this Structure==
3D8D is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3D8D OCA].  
3D8D is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3D8D OCA].  


==Reference==
==Reference==
Crystal structure of the human Fe65-PTB1 domain., Radzimanowski J, Ravaud S, Schlesinger S, Koch J, Beyreuther K, Sinning I, Wild K, J Biol Chem. 2008 Aug 22;283(34):23113-20. Epub 2008 Jun 11. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18550529 18550529]
Mercury-induced crystallization and SAD phasing of the human Fe65-PTB1 domain., Radzimanowski J, Ravaud S, Beyreuther K, Sinning I, Wild K, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 May 1;64(Pt, 5):382-5. Epub 2008 Apr 5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18453707 18453707]
Mercury-induced crystallization and SAD phasing of the human Fe65-PTB1 domain., Radzimanowski J, Ravaud S, Beyreuther K, Sinning I, Wild K, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 May 1;64(Pt, 5):382-5. Epub 2008 Apr 5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18453707 18453707]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Radzimanowski, J.]]
[[Category: Radzimanowski, J.]]
[[Category: Ravaud, S.]]
[[Category: Ravaud, S.]]
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[[Category: Polymorphism]]
[[Category: Polymorphism]]
[[Category: Protein binding]]
[[Category: Protein binding]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 11 10:51:53 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Nov 19 19:05:59 2008''

Revision as of 17:06, 19 November 2008

File:3d8d.png

Template:STRUCTURE 3d8d

Crystal structure of the human Fe65-PTB1 domain

Template:ABSTRACT PUBMED 18550529

About this Structure

3D8D is a 2 chains structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Crystal structure of the human Fe65-PTB1 domain., Radzimanowski J, Ravaud S, Schlesinger S, Koch J, Beyreuther K, Sinning I, Wild K, J Biol Chem. 2008 Aug 22;283(34):23113-20. Epub 2008 Jun 11. PMID:18550529

Mercury-induced crystallization and SAD phasing of the human Fe65-PTB1 domain., Radzimanowski J, Ravaud S, Beyreuther K, Sinning I, Wild K, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 May 1;64(Pt, 5):382-5. Epub 2008 Apr 5. PMID:18453707

Page seeded by OCA on Wed Nov 19 19:05:59 2008

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