1ca5: Difference between revisions

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[[Image:1ca5.gif|left|200px]]<br /><applet load="1ca5" size="~~450~~" color="white" frame="true" align="right" spinBox="true"

[[Image:1ca5.gif|left|200px]]<br /><applet load="1ca5" size="350" color="white" frame="true" align="right" spinBox="true"

caption="1ca5, resolution 2.2Å" />

'''INTERCALATION SITE OF HYPERTHERMOPHILE CHROMOSOMAL PROTEIN SSO7D/SAC7D BOUND TO DNA'''<br />

==Overview==

Sso7d and Sac7d are two small chromatin proteins from the, hyperthermophilic archaeabacterium Sulfolobus solfataricus and Sulfolobus, acidocaldarius, respectively. The crystal structures of Sso7d-GTGATCGC, Sac7d-GTGATCGC and Sac7d-GTGATCAC have been determined and refined at 1.45, A, 2.2 A and 2.2 A, respectively, to investigate the DNA binding property, of Sso7d/Sac7d in the presence of a T-G mismatch base-pair. Detailed, structural analysis revealed that the intercalation site includes the T-G, mismatch base-pair and Sso7d/Sac7d bind to that mismatch base-pair in a, manner similar to regular DNA. In the Sso7d-GTGATCGC complex, a new, inter-strand hydrogen bond between T2O4 and C14N4 is formed and well-order, bridging water molecules are found. The results suggest that the less, stable DNA stacking site involving a T-G mismatch may be a preferred site, for protein side-chain intercalation.

Sso7d and Sac7d are two small chromatin proteins from the hyperthermophilic archaeabacterium Sulfolobus solfataricus and Sulfolobus acidocaldarius, respectively. The crystal structures of Sso7d-GTGATCGC, Sac7d-GTGATCGC and Sac7d-GTGATCAC have been determined and refined at 1.45 A, 2.2 A and 2.2 A, respectively, to investigate the DNA binding property of Sso7d/Sac7d in the presence of a T-G mismatch base-pair. Detailed structural analysis revealed that the intercalation site includes the T-G mismatch base-pair and Sso7d/Sac7d bind to that mismatch base-pair in a manner similar to regular DNA. In the Sso7d-GTGATCGC complex, a new inter-strand hydrogen bond between T2O4 and C14N4 is formed and well-order bridging water molecules are found. The results suggest that the less stable DNA stacking site involving a T-G mismatch may be a preferred site for protein side-chain intercalation.

==About this Structure==

1CA5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sulfolobus_acidocaldarius Sulfolobus acidocaldarius]. Full crystallographic information is available from [http://~~ispc~~.weizmann.ac.il/oca-bin/ocashort?id=1CA5 OCA].

1CA5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sulfolobus_acidocaldarius Sulfolobus acidocaldarius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CA5 OCA].

==Reference==

Line 13:

[[Category: Single protein]]

[[Category: Sulfolobus acidocaldarius]]

[[Category: Gao, Y.G.]]

[[Category: Gao, Y G.]]

[[Category: Robinson, H.]]

[[Category: Shriver, J.W.]]

[[Category: Shriver, J W.]]

[[Category: Su, S.]]

[[Category: Wang, A.H.J.]]

[[Category: Wang, A H.J.]]

[[Category: chromosomal protein]]

[[Category: dna binding]]

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[[Category: sso7d]]

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@@ Line 1: / Line 1: @@
-[[Image:1ca5.gif|left|200px]]<br /><applet load="1ca5" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:1ca5.gif|left|200px]]<br /><applet load="1ca5" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="1ca5, resolution 2.2&Aring;" />
 '''INTERCALATION SITE OF HYPERTHERMOPHILE CHROMOSOMAL PROTEIN SSO7D/SAC7D BOUND TO DNA'''<br />
 ==Overview==
-Sso7d and Sac7d are two small chromatin proteins from the, hyperthermophilic archaeabacterium Sulfolobus solfataricus and Sulfolobus, acidocaldarius, respectively. The crystal structures of Sso7d-GTGATCGC, Sac7d-GTGATCGC and Sac7d-GTGATCAC have been determined and refined at 1.45, A, 2.2 A and 2.2 A, respectively, to investigate the DNA binding property, of Sso7d/Sac7d in the presence of a T-G mismatch base-pair. Detailed, structural analysis revealed that the intercalation site includes the T-G, mismatch base-pair and Sso7d/Sac7d bind to that mismatch base-pair in a, manner similar to regular DNA. In the Sso7d-GTGATCGC complex, a new, inter-strand hydrogen bond between T2O4 and C14N4 is formed and well-order, bridging water molecules are found. The results suggest that the less, stable DNA stacking site involving a T-G mismatch may be a preferred site, for protein side-chain intercalation.
+Sso7d and Sac7d are two small chromatin proteins from the hyperthermophilic archaeabacterium Sulfolobus solfataricus and Sulfolobus acidocaldarius, respectively. The crystal structures of Sso7d-GTGATCGC, Sac7d-GTGATCGC and Sac7d-GTGATCAC have been determined and refined at 1.45 A, 2.2 A and 2.2 A, respectively, to investigate the DNA binding property of Sso7d/Sac7d in the presence of a T-G mismatch base-pair. Detailed structural analysis revealed that the intercalation site includes the T-G mismatch base-pair and Sso7d/Sac7d bind to that mismatch base-pair in a manner similar to regular DNA. In the Sso7d-GTGATCGC complex, a new inter-strand hydrogen bond between T2O4 and C14N4 is formed and well-order bridging water molecules are found. The results suggest that the less stable DNA stacking site involving a T-G mismatch may be a preferred site for protein side-chain intercalation.
 ==About this Structure==
-CA5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sulfolobus_acidocaldarius Sulfolobus acidocaldarius]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1CA5 OCA].
+CA5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sulfolobus_acidocaldarius Sulfolobus acidocaldarius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CA5 OCA].
 ==Reference==
@@ Line 13: / Line 13: @@
 [[Category: Single protein]]
 [[Category: Sulfolobus acidocaldarius]]
-[[Category: Gao, Y.G.]]
+[[Category: Gao, Y G.]]
 [[Category: Robinson, H.]]
-[[Category: Shriver, J.W.]]
+[[Category: Shriver, J W.]]
 [[Category: Su, S.]]
-[[Category: Wang, A.H.J.]]
+[[Category: Wang, A H.J.]]
 [[Category: chromosomal protein]]
 [[Category: dna binding]]
@@ Line 24: / Line 24: @@
 [[Category: sso7d]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 12:18:01 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:04:01 2008''