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| [[Image:2vrh.jpg|left|200px]] | | {{Seed}} |
| | [[Image:2vrh.png|left|200px]] |
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| {{STRUCTURE_2vrh| PDB=2vrh | SCENE= }} | | {{STRUCTURE_2vrh| PDB=2vrh | SCENE= }} |
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| '''STRUCTURE OF THE E. COLI TRIGGER FACTOR BOUND TO A TRANSLATING RIBOSOME'''
| | ===STRUCTURE OF THE E. COLI TRIGGER FACTOR BOUND TO A TRANSLATING RIBOSOME=== |
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| ==Overview==
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| Ribosome-associated chaperone Trigger Factor (TF) initiates folding of newly synthesized proteins in bacteria. Here, we pinpoint by site-specific crosslinking the sequence of molecular interactions of Escherichia coli TF and nascent chains during translation. Furthermore, we provide the first full-length structure of TF associated with ribosome-nascent chain complexes by using cryo-electron microscopy. In its active state, TF arches over the ribosomal exit tunnel accepting nascent chains in a protective void. The growing nascent chain initially follows a predefined path through the entire interior of TF in an unfolded conformation, and even after folding into a domain it remains accommodated inside the protective cavity of ribosome-bound TF. The adaptability to accept nascent chains of different length and folding states may explain how TF is able to assist co-translational folding of all kinds of nascent polypeptides during ongoing synthesis. Moreover, we suggest a model of how TF's chaperoning function can be coordinated with the co-translational processing and membrane targeting of nascent polypeptides by other ribosome-associated factors.
| | The line below this paragraph, {{ABSTRACT_PUBMED_18497744}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 18497744 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_18497744}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Rrna-binding]] | | [[Category: Rrna-binding]] |
| [[Category: Trigger factor]] | | [[Category: Trigger factor]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 18 12:05:56 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 08:20:26 2008'' |
